Nucleolin and fibrillarin expression in stimulated lymphocytes and differentiating HL-60 cells. A flow cytometric assay

G. Mehes, L. Pajor

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Nucleolin and fibrillarin are two histone-like major proteins in the nucleolus that were found to be overexpressed in proliferating cells. Using specific antibodies to either nucleolin or fibrillarin now cytometric, measurements were carried out to demonstrate quantitative changes of these proteins during lymphocyte mitogenic activation and differentiation of HL-60 promyelocytic leukaemia cells. The expression of nucleolin increased during lymphocyte stimulation and decreased slowly but constantly in the course of differentiation of HL-60 cells. Expression of fibrillarin reached a maximum in the first cell cycle and then dropped to a basic level in stimulated lymphocytes. Compared to nucleolin, the level of fibrillarin decreased more rapidly and more extensively in differentiating HL-60 cells. The data support other observations that nucleolin is a stabile structural protein at the ribosomal genes while fibrillarin may have a more specific functional role in nucleologenesis and ribosome production.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalCell Proliferation
Volume28
Issue number6
Publication statusPublished - 1995

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HL-60 Cells
Lymphocytes
Lymphocyte Activation
Ribosomal Proteins
Ribosomes
Histones
Cell Cycle
Leukemia
Proteins
nucleolin
fibrillarin
Antibodies
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nucleolin and fibrillarin expression in stimulated lymphocytes and differentiating HL-60 cells. A flow cytometric assay. / Mehes, G.; Pajor, L.

In: Cell Proliferation, Vol. 28, No. 6, 1995, p. 329-336.

Research output: Contribution to journalArticle

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