Nucleocytoplasmic partitioning of the plant photoreceptors phytochrome A, B, C, D, and E is regulated differentially by light and exhibits a diurnal rhythm

Stefan Kircher, Patricia Gil, L. Kozma-Bognár, Erzsébet Fejes, Volker Speth, Tania Husselstein-Muller, Diana Bauer, E. Ádám, Eberhard Schäfer, Ferenc Nagy

Research output: Contribution to journalArticle

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Abstract

The phytochrome family of plant photoreceptors has a central role in the adaptation of plant development to changes in ambient light conditions. The individual phytochrome species regulate different or partly overlapping physiological responses. We generated transgenic Arabidopsis plants expressing phytochrome A to E:green fluorescent protein (GFP) fusion proteins to assess the biological role of intracellular compartmentation of these photoreceptors in light-regulated signaling. We show that all phytochrome:GFP fusion proteins were imported into the nuclei. Translocation of these photoreceptors into the nuclei was regulated differentially by light. Light-induced accumulation of phytochrome species in the nuclei resulted in the formation of speckles. The appearance of these nuclear structures exhibited distinctly different kinetics, wavelengths, and fluence dependence and was regulated by a diurnal rhythm. Furthermore, we demonstrate that the import of mutant phytochrome B:GFP and phytochrome A:GFP fusion proteins, shown to be defective in signaling in vivo, is regulated by light but is not accompanied by the formation of speckles. These results suggest that (1) the differential regulation of the translocation of phytochrome A to E into nuclei plays a role in the specification of functions, and (2) the appearance of speckles is a functional feature of phytochrome-regulated signaling.

Original languageEnglish
Pages (from-to)1541-1555
Number of pages15
JournalPlant Cell
Volume14
Issue number7
DOIs
Publication statusPublished - 2002

Fingerprint

Plant Photoreceptors
Phytochrome B
Phytochrome A
Phytochrome
phytochrome
Circadian Rhythm
photoreceptors
circadian rhythm
Green Fluorescent Proteins
Light
Speckle
Fusion reactions
green fluorescent protein
Proteins
Plant Development
Genetically Modified Plants
Staphylococcal Protein A
Arabidopsis
Specifications
proteins

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Nucleocytoplasmic partitioning of the plant photoreceptors phytochrome A, B, C, D, and E is regulated differentially by light and exhibits a diurnal rhythm. / Kircher, Stefan; Gil, Patricia; Kozma-Bognár, L.; Fejes, Erzsébet; Speth, Volker; Husselstein-Muller, Tania; Bauer, Diana; Ádám, E.; Schäfer, Eberhard; Nagy, Ferenc.

In: Plant Cell, Vol. 14, No. 7, 2002, p. 1541-1555.

Research output: Contribution to journalArticle

Kircher, Stefan ; Gil, Patricia ; Kozma-Bognár, L. ; Fejes, Erzsébet ; Speth, Volker ; Husselstein-Muller, Tania ; Bauer, Diana ; Ádám, E. ; Schäfer, Eberhard ; Nagy, Ferenc. / Nucleocytoplasmic partitioning of the plant photoreceptors phytochrome A, B, C, D, and E is regulated differentially by light and exhibits a diurnal rhythm. In: Plant Cell. 2002 ; Vol. 14, No. 7. pp. 1541-1555.
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