Nuclear localization of a hypoxia-inducible novel non-symbiotic hemoglobin in cultured alfalfa cells

Csaba Seregélyes, László Mustárdy, Ferhan Ayaydin, László Sass, László Kovács, Gabriella Endre, Noémi Lukács, Izabella Kovács, Imre Vass, György B. Kiss, Gábor V. Horváth, Dénes Dudits

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We have isolated a 483-bp-long full-length cDNA clone encoding a non-symbiotic hemoglobin called Mhb1, the first one found in alfalfa. This non-symbiotic hemoglobin is a single copy gene localized in linkage group 4 in diploid Medicago genome. The Mhb1 mRNA was found only in the roots of alfalfa plants. The Mhb1 gene was inducible by hypoxia and showed no induction by cold stress treatment. The Mhb1 transcript level increased at the G2/M boundary in a synchronized alfalfa cell suspension culture. The majority of Mhb1 protein was shown to be localized in the nucleus and smaller amounts were detected in the cytoplasm. A potential link to the nitric oxide signalling pathway is also discussed. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalFEBS letters
Issue number1-2
Publication statusPublished - Sep 29 2000



  • Cell division cycle
  • Hypoxia
  • Immunolocalization
  • Non-symbiotic hemoglobin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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