Nuclear factor κB activation in a type V pityriasis rubra pilaris patient harboring multiple CARD14 variants

Judit Danis, Anikó Göblös, Brigitta Gál, Adrienn Sulák, Katalin Farkas, Dóra Török, Erika Varga, Irma Korom, Lajos Kemény, Márta Széll, Zsuzsanna Bata-Csörgő, Nikoletta Nagy

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Abstract

Pityriasis rubra pilaris (PRP) is a rare papulosquamous skin disorder, which is pheno-typically related to psoriasis. Some familial PRP cases show autosomal dominant inheritance due to CARD14 mutations leading to increased nuclear factor κB (NFκB) activation. Moreover, CARD14 polymorphisms have also been implicated in sporadic PRP. A Hungarian PRP patient with childhood onset disease showing worsening of the symptoms in adulthood with poor therapeutic response underwent genetic screening for the CARD14 gene, revealing four genetic variants (rs117918077, rs2066964, rs28674001, and rs11652075). To confirm that the identified genetic variants would result in altered NFκB activity in the patient, functional studies were carried out. Immunofluorescent staining of the NFκB p65 subunit and NFκB-luciferase reporter assay demonstrated significantly increased NFκB activity in skin samples and keratinocytes from the PRP patient compared to healthy samples. Characterization of the cytokine profile of the keratinocytes and peripheral blood mononuclear cells demonstrated that the higher NFκB activation in PRP cells induces enhanced responses to inflammatory stimuli. These higher inflammatory reactions could not be explained solely by the observed CARD14 or other inflammation-related gene variants (determined by whole exome sequencing). Thus our study indicates the importance of investigations on other genetic factors related to PRP and their further functional characterization to bring us closer to the understanding of cellular and molecular background of disease pathogenesis.

Original languageEnglish
Article number1564
JournalFrontiers in immunology
Volume9
DOIs
Publication statusPublished - Jul 2018

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Keywords

  • CARD14 gene
  • Inflammation
  • Nuclear factor κB signaling pathway
  • Pityriasis rubra pilaris
  • Psoriasis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Danis, J., Göblös, A., Gál, B., Sulák, A., Farkas, K., Török, D., Varga, E., Korom, I., Kemény, L., Széll, M., Bata-Csörgő, Z., & Nagy, N. (2018). Nuclear factor κB activation in a type V pityriasis rubra pilaris patient harboring multiple CARD14 variants. Frontiers in immunology, 9, [1564]. https://doi.org/10.3389/fimmu.2018.01564