Noxious heat activates all capsaicin-sensitive and also a sub-population of capsaicin-insensitive dorsal root ganglion neurons

I. Nagy, H. Rang

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

A sub-population of primary afferent fibres comprising mainly Aδ and C polymodal nociceptors specifically detects high intensity heat stimuli. These fibres are also sensitive to high threshold mechanical stimulation and different chemicals including inflammatory mediators. C-polymodal nociceptors are also activated by capsaicin. Recent findings show that noxious heat induces inward currents in a sub-population of cultured dorsal root ganglion neurons by opening nonselective cation-channels. It has been suggested that noxious heat is transduced by the recently cloned capsaicin-gated ion- channel since oocytes and HEK 293 cells expressing this channel respond to heat as well as capsaicin. In agreement with this suggestion Kirschstein et al. found in a small sample of dorsal root ganglion cells that all heat- sensitive neurons were also sensitive to capsaicin. In this study we examine further, by whole-cell voltage-clamp recording from adult rat dorsal root ganglion neurons grown in culture, the relationship between heat- and capsaicin-sensitivity. Our results show the existence of two kinds of heat- sensitive neurons, distinguished by their temperature thresholds. The low- threshold cells, which comprise the small-medium diameter population, are capsaicin-sensitive, whereas the high-threshold (mainly large-diameter) cells are not, and we postulate the existence of a heat transducer distinct from capsaicin receptor in the latter group.

Original languageEnglish
Pages (from-to)995-997
Number of pages3
JournalNeuroscience
Volume88
Issue number4
DOIs
Publication statusPublished - Feb 1999

Fingerprint

Capsaicin
Spinal Ganglia
Hot Temperature
Neurons
Population
Nociceptors
Chemical Stimulation
TRPV Cation Channels
HEK293 Cells
Vulnerable Populations
Transducers
Ion Channels
Oocytes
Cations
Temperature

Keywords

  • Capsaicin
  • Noxious heat threshold
  • Sensory neurons
  • Thermosensitivity
  • Whole-cell voltage-clamp

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Noxious heat activates all capsaicin-sensitive and also a sub-population of capsaicin-insensitive dorsal root ganglion neurons. / Nagy, I.; Rang, H.

In: Neuroscience, Vol. 88, No. 4, 02.1999, p. 995-997.

Research output: Contribution to journalArticle

@article{62bd7114301e4e7ebc2c63dab8df22a0,
title = "Noxious heat activates all capsaicin-sensitive and also a sub-population of capsaicin-insensitive dorsal root ganglion neurons",
abstract = "A sub-population of primary afferent fibres comprising mainly Aδ and C polymodal nociceptors specifically detects high intensity heat stimuli. These fibres are also sensitive to high threshold mechanical stimulation and different chemicals including inflammatory mediators. C-polymodal nociceptors are also activated by capsaicin. Recent findings show that noxious heat induces inward currents in a sub-population of cultured dorsal root ganglion neurons by opening nonselective cation-channels. It has been suggested that noxious heat is transduced by the recently cloned capsaicin-gated ion- channel since oocytes and HEK 293 cells expressing this channel respond to heat as well as capsaicin. In agreement with this suggestion Kirschstein et al. found in a small sample of dorsal root ganglion cells that all heat- sensitive neurons were also sensitive to capsaicin. In this study we examine further, by whole-cell voltage-clamp recording from adult rat dorsal root ganglion neurons grown in culture, the relationship between heat- and capsaicin-sensitivity. Our results show the existence of two kinds of heat- sensitive neurons, distinguished by their temperature thresholds. The low- threshold cells, which comprise the small-medium diameter population, are capsaicin-sensitive, whereas the high-threshold (mainly large-diameter) cells are not, and we postulate the existence of a heat transducer distinct from capsaicin receptor in the latter group.",
keywords = "Capsaicin, Noxious heat threshold, Sensory neurons, Thermosensitivity, Whole-cell voltage-clamp",
author = "I. Nagy and H. Rang",
year = "1999",
month = "2",
doi = "10.1016/S0306-4522(98)00535-1",
language = "English",
volume = "88",
pages = "995--997",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Noxious heat activates all capsaicin-sensitive and also a sub-population of capsaicin-insensitive dorsal root ganglion neurons

AU - Nagy, I.

AU - Rang, H.

PY - 1999/2

Y1 - 1999/2

N2 - A sub-population of primary afferent fibres comprising mainly Aδ and C polymodal nociceptors specifically detects high intensity heat stimuli. These fibres are also sensitive to high threshold mechanical stimulation and different chemicals including inflammatory mediators. C-polymodal nociceptors are also activated by capsaicin. Recent findings show that noxious heat induces inward currents in a sub-population of cultured dorsal root ganglion neurons by opening nonselective cation-channels. It has been suggested that noxious heat is transduced by the recently cloned capsaicin-gated ion- channel since oocytes and HEK 293 cells expressing this channel respond to heat as well as capsaicin. In agreement with this suggestion Kirschstein et al. found in a small sample of dorsal root ganglion cells that all heat- sensitive neurons were also sensitive to capsaicin. In this study we examine further, by whole-cell voltage-clamp recording from adult rat dorsal root ganglion neurons grown in culture, the relationship between heat- and capsaicin-sensitivity. Our results show the existence of two kinds of heat- sensitive neurons, distinguished by their temperature thresholds. The low- threshold cells, which comprise the small-medium diameter population, are capsaicin-sensitive, whereas the high-threshold (mainly large-diameter) cells are not, and we postulate the existence of a heat transducer distinct from capsaicin receptor in the latter group.

AB - A sub-population of primary afferent fibres comprising mainly Aδ and C polymodal nociceptors specifically detects high intensity heat stimuli. These fibres are also sensitive to high threshold mechanical stimulation and different chemicals including inflammatory mediators. C-polymodal nociceptors are also activated by capsaicin. Recent findings show that noxious heat induces inward currents in a sub-population of cultured dorsal root ganglion neurons by opening nonselective cation-channels. It has been suggested that noxious heat is transduced by the recently cloned capsaicin-gated ion- channel since oocytes and HEK 293 cells expressing this channel respond to heat as well as capsaicin. In agreement with this suggestion Kirschstein et al. found in a small sample of dorsal root ganglion cells that all heat- sensitive neurons were also sensitive to capsaicin. In this study we examine further, by whole-cell voltage-clamp recording from adult rat dorsal root ganglion neurons grown in culture, the relationship between heat- and capsaicin-sensitivity. Our results show the existence of two kinds of heat- sensitive neurons, distinguished by their temperature thresholds. The low- threshold cells, which comprise the small-medium diameter population, are capsaicin-sensitive, whereas the high-threshold (mainly large-diameter) cells are not, and we postulate the existence of a heat transducer distinct from capsaicin receptor in the latter group.

KW - Capsaicin

KW - Noxious heat threshold

KW - Sensory neurons

KW - Thermosensitivity

KW - Whole-cell voltage-clamp

UR - http://www.scopus.com/inward/record.url?scp=0032889729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032889729&partnerID=8YFLogxK

U2 - 10.1016/S0306-4522(98)00535-1

DO - 10.1016/S0306-4522(98)00535-1

M3 - Article

C2 - 10336113

AN - SCOPUS:0032889729

VL - 88

SP - 995

EP - 997

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 4

ER -