Novel type of flow injection immunoassay for the determination of phenytoin in serum

Andrea Bereczki, Viola Horváth

Research output: Contribution to journalArticle

14 Citations (Scopus)


A novel flow injection immunoassay using fluorimetric detection successfully measured an antiepileptic drug, phenytoin, from a serum background. The assay was based on the competitive binding of fluorescein labelled and unlabelled phenytoin followed by the separation of bound and unbound antigens with gel filtration chromatography. The immunoreaction and subsequent detection of products was performed in a newly designed stopped flow injection system employing a simple two injector system for reagent injection, mixing and rigorous timing of the incubation. Two experimental set-ups were tested. The optimal parameters and configurations of the systems were established and the results were verified against an independent method. The calibration curves were linear showing good sensitivity and reproducibility in the concentration range required for clinical analyses suggesting that this technique could be a viable alternative for the measurement of the phenytoin in serum samples. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)9-17
Number of pages9
JournalAnalytica Chimica Acta
Issue number1
Publication statusPublished - May 26 1999


  • Flow injection immunoassay
  • Fluorescence
  • Phenytoin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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