Novel tracing paradigms - Genetically engineered herpesviruses as tools for mapping functional circuits within the CNS: Present status and future prospects

Zsolt Boldogköi, Attila Sík, Ádám Dénes, Anikó Reichart, József Toldi, Ida Gerendai, Krisztina J. Kovács, Miklós Palkovits

Research output: Contribution to journalReview article

60 Citations (Scopus)

Abstract

The mammalian CNS is composed of an extremely complex meshwork of highly ordered interconnections among billions of neurons. To understand the diverse functions of this neuronal network we need to differentiate between functionally related and nonrelated elements. A powerful labeling method for defining intricate neural circuits is based on the utilization of neurotropic herpesviruses, including pseudorabies virus and herpes simplex virus type 1. The recent development of genetically engineered tracing viruses can open the way toward the conception of novel tract-tracing paradigms. These new-generation tracing viruses may facilitate the clarification of problems, which were inaccessible to earlier approaches. This article first presents a concise review of the general aspects of neuroanatomical tracing protocols. Subsequently, it discusses the molecular biology of α-herpesviruses, and the genetic manipulation and gene expression techniques that are utilized for the construction of virus-based tracers. Finally, it describes the current utilization of genetically modified herpesviruses for circuit analysis, and the future directions in their potential applications.

Original languageEnglish
Pages (from-to)417-445
Number of pages29
JournalProgress in Neurobiology
Volume72
Issue number6
DOIs
Publication statusPublished - Apr 1 2004

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Keywords

  • Bartha strain of PRV
  • Becker strain of PRV
  • CMVP
  • HSV
  • ICP
  • IE
  • LAP
  • PRV
  • PRV-Ba
  • PRV-Be
  • PRV-Ka
  • herpes simplex virus
  • human cytomegalovirus IE1 promoter
  • immediate early
  • infected cell polypeptide
  • latency-associated promoter
  • pseudorabies virus

ASJC Scopus subject areas

  • Neuroscience(all)

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