Novel technologies applied to the nuoleotide sequencing and comparative sequence analvsis of the genomes of infectious agents in veterinary medicine

F. Granberg, A. Bálint, S. Belák

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Next-generation sequencing (NGS), also referred to as deep, high-throughput or massively parallel sequencing, is a powerful new tool that can be used for the complex diagnosis and intensive monitoring of infectious disease in veterinary medicine. NGS technologies are also being increasingly used to study the aetiology, genomics, evolution and epidemiology of infectious disease, as well as host-pathogen interactions and other aspects of infection biology. This review briefly summarises recent progress and achievements in this field by first introducing a range of novel techniques and then presenting examples of NGS applications in veterinary infection biology. Various work steps and processes for sampling and sample preparation, sequence analysis and comparative genomics, and improving the accuracy of genomic prediction are discussed, as are bioinformatics requirements. Examples of sequencing-based applications and comparative genomics in veterinary medicine are then provided. This review is based on novel references selected from the literature and on experiences of the World Organisation for Animal Health (OIE) Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine, Uppsala, Sweden.

Original languageEnglish
Pages (from-to)25-42
Number of pages18
JournalOIE Revue Scientifique et Technique
Volume35
Issue number1
DOIs
Publication statusPublished - Apr 1 2016

Fingerprint

veterinary medicine
infectious diseases
genomics
genome
pathogens
Biological Sciences
host-pathogen relationships
bioinformatics
animal health
infection
biotechnology
etiology
epidemiology
Sweden
sequence analysis
sampling
prediction
monitoring
methodology

Keywords

  • Bacteria
  • Comparative genomics
  • Diagnosis
  • High-throughput sequencing
  • Infectious agent
  • Infectious disease
  • Metagenomics
  • Next-generation sequencing
  • Veterinary medicine
  • Virus

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

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