Novel steroid derivative modulates gene expression of cytokines and growth regulators

G. Michel, K. Nowok, A. Beetz, C. Ried, L. Kemény, T. Ruzicka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Topical steroid treatment is a common therapy for psoriasis. Steroids are known to bind to specific cytoplasmic receptors and to influence gene expression. We investigated the effects of the novel steroid derivative mometasone furoate on the expression of putative target genes in normal human epidermal cells (KC). Gene expression was measured by semiquantitative mRNA-PCR. In addition, cytokine receptor characteristics were assessed by ligand binding studies. We found a dose-dependent downregulation of proinflammatory mediators (IL-8, TNFα). Genes involved in growth regulation (HER-2, p53) were also modulated. IL-8 binding to KC was inhibited. We conclude that modulation of the expression of cytokine, cytokine receptor and growth factor genes may contribute to the antipsoriatic action of steroids.

Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalSkin Pharmacology
Volume8
Issue number5
Publication statusPublished - 1995

Fingerprint

Steroids
Cytokines
Gene Expression
Cytokine Receptors
Mometasone Furoate
Growth
Interleukin-8
Genes
Cytoplasmic and Nuclear Receptors
Psoriasis
Intercellular Signaling Peptides and Proteins
Down-Regulation
Ligands
Polymerase Chain Reaction
Messenger RNA
Therapeutics

Keywords

  • HER-2
  • IL-8
  • p53
  • Psoriasis
  • Steroids
  • TNFα

ASJC Scopus subject areas

  • Dermatology
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Michel, G., Nowok, K., Beetz, A., Ried, C., Kemény, L., & Ruzicka, T. (1995). Novel steroid derivative modulates gene expression of cytokines and growth regulators. Skin Pharmacology, 8(5), 215-220.

Novel steroid derivative modulates gene expression of cytokines and growth regulators. / Michel, G.; Nowok, K.; Beetz, A.; Ried, C.; Kemény, L.; Ruzicka, T.

In: Skin Pharmacology, Vol. 8, No. 5, 1995, p. 215-220.

Research output: Contribution to journalArticle

Michel, G, Nowok, K, Beetz, A, Ried, C, Kemény, L & Ruzicka, T 1995, 'Novel steroid derivative modulates gene expression of cytokines and growth regulators', Skin Pharmacology, vol. 8, no. 5, pp. 215-220.
Michel, G. ; Nowok, K. ; Beetz, A. ; Ried, C. ; Kemény, L. ; Ruzicka, T. / Novel steroid derivative modulates gene expression of cytokines and growth regulators. In: Skin Pharmacology. 1995 ; Vol. 8, No. 5. pp. 215-220.
@article{304560cb8ffb4e679d094509c661e868,
title = "Novel steroid derivative modulates gene expression of cytokines and growth regulators",
abstract = "Topical steroid treatment is a common therapy for psoriasis. Steroids are known to bind to specific cytoplasmic receptors and to influence gene expression. We investigated the effects of the novel steroid derivative mometasone furoate on the expression of putative target genes in normal human epidermal cells (KC). Gene expression was measured by semiquantitative mRNA-PCR. In addition, cytokine receptor characteristics were assessed by ligand binding studies. We found a dose-dependent downregulation of proinflammatory mediators (IL-8, TNFα). Genes involved in growth regulation (HER-2, p53) were also modulated. IL-8 binding to KC was inhibited. We conclude that modulation of the expression of cytokine, cytokine receptor and growth factor genes may contribute to the antipsoriatic action of steroids.",
keywords = "HER-2, IL-8, p53, Psoriasis, Steroids, TNFα",
author = "G. Michel and K. Nowok and A. Beetz and C. Ried and L. Kem{\'e}ny and T. Ruzicka",
year = "1995",
language = "English",
volume = "8",
pages = "215--220",
journal = "Skin Pharmacology and Physiology",
issn = "1660-5527",
publisher = "S. Karger AG",
number = "5",

}

TY - JOUR

T1 - Novel steroid derivative modulates gene expression of cytokines and growth regulators

AU - Michel, G.

AU - Nowok, K.

AU - Beetz, A.

AU - Ried, C.

AU - Kemény, L.

AU - Ruzicka, T.

PY - 1995

Y1 - 1995

N2 - Topical steroid treatment is a common therapy for psoriasis. Steroids are known to bind to specific cytoplasmic receptors and to influence gene expression. We investigated the effects of the novel steroid derivative mometasone furoate on the expression of putative target genes in normal human epidermal cells (KC). Gene expression was measured by semiquantitative mRNA-PCR. In addition, cytokine receptor characteristics were assessed by ligand binding studies. We found a dose-dependent downregulation of proinflammatory mediators (IL-8, TNFα). Genes involved in growth regulation (HER-2, p53) were also modulated. IL-8 binding to KC was inhibited. We conclude that modulation of the expression of cytokine, cytokine receptor and growth factor genes may contribute to the antipsoriatic action of steroids.

AB - Topical steroid treatment is a common therapy for psoriasis. Steroids are known to bind to specific cytoplasmic receptors and to influence gene expression. We investigated the effects of the novel steroid derivative mometasone furoate on the expression of putative target genes in normal human epidermal cells (KC). Gene expression was measured by semiquantitative mRNA-PCR. In addition, cytokine receptor characteristics were assessed by ligand binding studies. We found a dose-dependent downregulation of proinflammatory mediators (IL-8, TNFα). Genes involved in growth regulation (HER-2, p53) were also modulated. IL-8 binding to KC was inhibited. We conclude that modulation of the expression of cytokine, cytokine receptor and growth factor genes may contribute to the antipsoriatic action of steroids.

KW - HER-2

KW - IL-8

KW - p53

KW - Psoriasis

KW - Steroids

KW - TNFα

UR - http://www.scopus.com/inward/record.url?scp=0029093872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029093872&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 215

EP - 220

JO - Skin Pharmacology and Physiology

JF - Skin Pharmacology and Physiology

SN - 1660-5527

IS - 5

ER -