Novel spin-labels for the study of lipid-protein interactions. Application to (Na+,K+)-ATPase membranes

Mikael Esmann, K. Hideg, Derek Marsh

Research output: Contribution to journalArticle

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Abstract

The interactions of a series of spin-labeled fatty acids, in which the nitroxide ring is incorporated in different ways as an integral part of the hydrocarbon chain, with the (Na+,K+)-ATPase in membranes from Squalus acanthias, have been studied by electron spin resonance spectroscopy. The fatty acids are 2,4-, 2,5-, and 3,2-substituents of 2,2,5,5-tetramethylpyrrolidine-N-oxyl and belong to the class of minimal perturbation nitroxide probes. For all five fatty acid labels, a motionally restricted lipid component was observed in the ESR spectra of (Na+,K+)-ATPase membranes, in addition to the fluid component, which was found in the spectra of the extracted membrane lipids. The pH dependence of the motionally restricted spin-label population indicated a sensitivity in the selectivity of the lipid-protein interaction to the protonation state of the fatty acid. These results agree with those found previously for the conventional oxazolidine (doxyl) fatty acid and phospholipid spin-label derivatives [Esmann, M., Watts, A., & Marsh, D. (1985) Biochemistry 24, 1386-1393] and indicate that the motion of the lipid chains is significantly hindered by interaction with the protein, irrespective of the nature of the spin-label group.

Original languageEnglish
Pages (from-to)3913-3917
Number of pages5
JournalBiochemistry
Volume27
Issue number11
Publication statusPublished - 1988

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Spin Labels
Adenosine Triphosphatases
Fatty Acids
Membranes
Lipids
Proteins
Squalus acanthias
Electron spin resonance spectroscopy
Biochemistry
Wetlands
Protonation
Electron Spin Resonance Spectroscopy
Membrane Lipids
Hydrocarbons
Paramagnetic resonance
Labels
Phospholipids
sodium-translocating ATPase
Derivatives
Fluids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Novel spin-labels for the study of lipid-protein interactions. Application to (Na+,K+)-ATPase membranes. / Esmann, Mikael; Hideg, K.; Marsh, Derek.

In: Biochemistry, Vol. 27, No. 11, 1988, p. 3913-3917.

Research output: Contribution to journalArticle

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