Novel sample preparation method for surfactant containing suppositories: Effect of micelle formation on drug recovery

Éva Kalmár, Konomi Ueno, Péter Forgó, Gerda Szakonyi, György Dombi

Research output: Contribution to journalArticle

7 Citations (Scopus)


Rectal drug delivery is currently at the focus of attention. Surfactants promote drug release from the suppository bases and enhance the formulation properties. The aim of our work was to develop a sample preparation method for HPLC analysis for a suppository base containing 95% hard fat, 2.5% Tween 20 and 2.5% Tween 60. A conventional sample preparation method did not provide successful results as the recovery of the drug failed to fulfil the validation criterion 95-105%. This was caused by the non-ionic surfactants in the suppository base incorporating some of the drug, preventing its release. As guidance for the formulation from an analytical aspect, we suggest a well defined surfactant content based on the turbidimetric determination of the CMC (critical micelle formation concentration) in the applied methanol-water solvent. Our CMC data correlate well with the results of previous studies. As regards the sample preparation procedure, a study was performed of the effects of ionic strength and pH on the drug recovery with the avoidance of degradation of the drug during the procedure. Aminophenazone and paracetamol were used as model drugs. The optimum conditions for drug release from the molten suppository base were found to be 100. mM NaCl, 20-40. mM NaOH and a 30. min ultrasonic treatment of the final sample solution. As these conditions could cause the degradation of the drugs in the solution, this was followed by NMR spectroscopy, and the results indicated that degradation did not take place. The determined CMCs were 0.08. mM for Tween 20, 0.06. mM for Tween 60 and 0.04. mM for a combined Tween 20, Tween 60 system.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Publication statusPublished - Sep 1 2013


  • HPLC
  • Micelle breaking
  • Suppository
  • Surfactants
  • Turbidimetry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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