Novel roles for murine complement receptors type 1 and 2. I. Regulation of B cell survival and proliferation by CR1/2

Eszter Molnár, A. Erdei, J. Prechl

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Innate components of the immune system, such as complement are known to have a modulatory effect on adaptive immune responses. Complement receptors are expressed by both B and T lymphocytes and play part in antigen presentation and cellular activation and adhesion events. On murine B cells type 1 and 2 complement receptors (CR1/2) are expressed and form a co-receptor complex together with CD19 and CD81. We used CR1/2 specific antibodies to assess the role these receptors might play in regulating cell cycling events of B cells. We show that a CR1/2 specific antibody fragment, 7G6 scFv can induce the proliferation of mature B cells. This effect is countermodulated by FcR crosslinkage and enhanced by BCR engagement. The proliferative effect is severely impaired in Cr2-/- animals, strengthening the involvement of CR1/2. Transitional B cells are prone to apoptotic death by selection events, yet they are rescued from apoptosis by CR1/2 crosslinkage. CR1/2 ligation by 7G6 scFv alone can induce nuclear translocation of NF-κB, supporting the above observations.We conclude that engagement of complement receptor 2 of B cells promotes the survival of both mature and transitional B cells. This activity supplements the previously described adjuvant effects of complement.

Original languageEnglish
Pages (from-to)156-162
Number of pages7
JournalImmunology Letters
Volume116
Issue number2
DOIs
Publication statusPublished - Mar 15 2008

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Complement 3d Receptors
Complement Receptors
Cell Survival
B-Lymphocytes
Cell Proliferation
B-Lymphoid Precursor Cells
Immunoglobulin Fragments
Antigen Presentation
Adaptive Immunity
Ligation
Immune System
Apoptosis
T-Lymphocytes
Antibodies

Keywords

  • Apoptosis
  • Complement
  • CR2
  • Proliferation
  • scFv

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Novel roles for murine complement receptors type 1 and 2. I. Regulation of B cell survival and proliferation by CR1/2. / Molnár, Eszter; Erdei, A.; Prechl, J.

In: Immunology Letters, Vol. 116, No. 2, 15.03.2008, p. 156-162.

Research output: Contribution to journalArticle

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