Novel quantitative electrophoretic analysis of endotoxins on microchips

Anikó Kilár, Viktor Farkas, Krisztina Kovács, Béla Kocsis, Ferenc Kilár

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A novel microchip electrophoresis method was developed and applied for sensitive detection and quantitative analysis of endotoxins extracted from Gram-negative bacteria. The method provides a fast and quantitative differentiation of smooth and rough endotoxins based on the solubilization and complexation of the lipopolysaccharides with dodecylsulfate, and then with a fluorescent dye. The migration of the complexes was followed by LIF detection. The novel method is able to replace the SDS-PAGE with the advantage of high speed and better sensitivity; and by avoiding the laborious gel-preparation and silver staining.

Original languageEnglish
Pages (from-to)1713-1722
Number of pages10
JournalELECTROPHORESIS
Volume29
Issue number8
DOIs
Publication statusPublished - Apr 1 2008

Keywords

  • Endotoxin
  • Lipopolysaccharide
  • Microchip electrophoresis
  • Sodium dodecyl sulfate polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Novel quantitative electrophoretic analysis of endotoxins on microchips'. Together they form a unique fingerprint.

  • Cite this