Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses

Adrian Peres, Michelle L. Churchman, Srivaidehirani Hariharan, Kristiina Himanen, Aurine Verkest, Klaas Vandepoele, Z. Magyar, Yves Hatzfeld, Els Van Der Schueren, Gerrit T S Beemster, Valerie Frankard, John C. Larkin, Dirk Inzé, Lieven De Veylder

Research output: Contribution to journalArticle

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Abstract

The EL2 gene of rice (Oryza sativa), previously classified as early response gene against the potent biotic elicitor N-acetylchitoheptaose and encoding a short polypeptide with unknown function, was identified as a novel cell cycle regulatory gene related to the recently reported SIAMESE (SIM) gene of Arabidopsis thaliana. Iterative two-hybrid screens, in vitro pull-down assays, and fluorescence resonance energy transfer analyses showed that Orysa; EL2 binds the cyclin-dependent kinase (CDK) CDKA1;1 and D-type cyclins. No interaction was observed with the plant-specific B-type CDKs. The amino acid motif ELERFL was identified to be essential for cyclin, but not for CDK binding. Orysa;EL2 impaired the ability of Orysa; CYCD5;3 to complement a budding yeast (Saccharomyces cerevisiae) triple CLN mutant, whereas recombinant protein inhibited CDK activity in vitro. Moreover, Orysa;EL2 was able to rescue the multicellular trichome phenotype of sim mutants of Arabidopsis, unequivocally demonstrating that Orysa;EL2 operates as a cell cycle inhibitor. Orysa;EL2 mRNA levels were induced by cold, drought, and propionic acid. Our data suggest that Orysa;EL2 encodes a new type of plant CDK inhibitor that links cell cycle progression with biotic and abiotic stress responses.

Original languageEnglish
Pages (from-to)25588-25596
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number35
DOIs
Publication statusPublished - Aug 31 2007

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Cyclin-Dependent Kinases
Genes
Arabidopsis
Cells
Cell Cycle
CDC2 Protein Kinase
Yeast
Cyclin D
Trichomes
cdc Genes
Fluorescence Resonance Energy Transfer
Amino Acid Motifs
Complement C3
Saccharomycetales
Cyclins
Droughts
Regulator Genes
Recombinant Proteins
Saccharomyces cerevisiae
Drought

ASJC Scopus subject areas

  • Biochemistry

Cite this

Peres, A., Churchman, M. L., Hariharan, S., Himanen, K., Verkest, A., Vandepoele, K., ... De Veylder, L. (2007). Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. Journal of Biological Chemistry, 282(35), 25588-25596. https://doi.org/10.1074/jbc.M703326200

Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. / Peres, Adrian; Churchman, Michelle L.; Hariharan, Srivaidehirani; Himanen, Kristiina; Verkest, Aurine; Vandepoele, Klaas; Magyar, Z.; Hatzfeld, Yves; Van Der Schueren, Els; Beemster, Gerrit T S; Frankard, Valerie; Larkin, John C.; Inzé, Dirk; De Veylder, Lieven.

In: Journal of Biological Chemistry, Vol. 282, No. 35, 31.08.2007, p. 25588-25596.

Research output: Contribution to journalArticle

Peres, A, Churchman, ML, Hariharan, S, Himanen, K, Verkest, A, Vandepoele, K, Magyar, Z, Hatzfeld, Y, Van Der Schueren, E, Beemster, GTS, Frankard, V, Larkin, JC, Inzé, D & De Veylder, L 2007, 'Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses', Journal of Biological Chemistry, vol. 282, no. 35, pp. 25588-25596. https://doi.org/10.1074/jbc.M703326200
Peres A, Churchman ML, Hariharan S, Himanen K, Verkest A, Vandepoele K et al. Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. Journal of Biological Chemistry. 2007 Aug 31;282(35):25588-25596. https://doi.org/10.1074/jbc.M703326200
Peres, Adrian ; Churchman, Michelle L. ; Hariharan, Srivaidehirani ; Himanen, Kristiina ; Verkest, Aurine ; Vandepoele, Klaas ; Magyar, Z. ; Hatzfeld, Yves ; Van Der Schueren, Els ; Beemster, Gerrit T S ; Frankard, Valerie ; Larkin, John C. ; Inzé, Dirk ; De Veylder, Lieven. / Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 35. pp. 25588-25596.
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