Novel, not adenylyl cyclase-coupled cannabinoid binding site in cerebellum of mice

Krisztina Monory, Eleni Th Tzavara, Jinette Lexime, Catherine Ledent, Marc Parmentier, Anna Borsodi, A. Borsodi

Research output: Contribution to journalArticle

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Abstract

In this study we report data suggesting the presence of a non-CB1, non-CB2 cannabinoid site in the cerebellum of CB1-/- mice. We have carried out [35S]GTPγS binding experiments in striata, hippocampi, and cerebella of CB1-/- and CB1+/+ mice with Δ9-THC, WIN55,212-2, HU-210, SR141716A, and SR144528. In CB1-/- mice Δ9-THC and HU-210 did not stimulate [35S]GTPγS binding. However, WIN55,212-2 was able to stimulate [35S]GTPγS binding in cerebella of CB1-/- mice. The maximal effect of this stimulation was 31% that of wild type animals. This effect was reversible neither by CB1 nor CB2 receptor antagonists. Similar results were obtained with the endogenous cannabinoid, anandamide. However, adenylyl cyclase was not inhibited by WIN55,212-2 or anandamide in the CB1-/- animals. In striata and hippocampi of CB1-/- mice no [35S]GTPγS stimulation curve could be obtained with WIN55,212. Our findings suggest that there is a non-CB1 non-CB2 receptor present in the cerebellum of CB1-/- mice.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume292
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

rimonabant
Dronabinol
Cannabinoids
Adenylyl Cyclases
Cerebellum
Animals
Binding Sites
Cannabinoid Receptor CB2
Hippocampus
Wild Animals
Experiments
HU 211
anandamide
SR 144528

Keywords

  • [S]GTPγS binding
  • Adenylyl cyclase
  • Cannabinoid receptor
  • CB1 knockout mice
  • Cerebellum

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Novel, not adenylyl cyclase-coupled cannabinoid binding site in cerebellum of mice. / Monory, Krisztina; Tzavara, Eleni Th; Lexime, Jinette; Ledent, Catherine; Parmentier, Marc; Borsodi, Anna; Borsodi, A.

In: Biochemical and Biophysical Research Communications, Vol. 292, No. 1, 2002, p. 231-235.

Research output: Contribution to journalArticle

Monory, Krisztina ; Tzavara, Eleni Th ; Lexime, Jinette ; Ledent, Catherine ; Parmentier, Marc ; Borsodi, Anna ; Borsodi, A. / Novel, not adenylyl cyclase-coupled cannabinoid binding site in cerebellum of mice. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 292, No. 1. pp. 231-235.
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