Novel method for induced propagation of fish

Sperm injection in oviducts and ovary/ovarian lavage with sperm

T. Müller, L. Horváth, T. Szabó, I. Ittzés, A. Bognár, P. Faidt, Ittzés, B. Urbányi, B. Kucska

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Motility of spermatozoa in most freshwater teleost species is suppressed by the osmolality of the seminal plasma in the testes, sperm ducts and initiated by a decrease of osmolality upon spawning in fresh water. We hypothesized that, similarly to internally fertilising fish species, spermatozoa of freshly stripped sperm, when injected directly into the ovary through the oviduct, will remain inactivated and maintain its fertilisation capacity for extended periods. Fertilisation is expected to occur after ovulated oocytes with spermatozoa on their surfaces are released into the water. To test this hypothesis, experiments were carried out on carp (Cyprinus carpio) as a model fish. Stripped and pooled sperm samples were injected by catheter into the ovarian cavity through the oviduct 2 h and 12 h before expected spawning. To prevent any loss of gametes the genital area of treated females were stitched by a standard protocol used in large-scale hatchery systems. At ovulation, eggs were stripped and fertilised with salt-carbamide solution. All sperm-injected females produced some fertilised eggs that developed normally while fertilisation rates varied considerably (24.2–81.2%). This proof of principle experiment demonstrates the potential for routine use as alternative to in vitro fertilisation for the propagation of fish species.

Original languageEnglish
Pages (from-to)124-129
Number of pages6
JournalAquaculture
Volume482
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

oviducts
sperm
spermatozoa
injection
fish
fertilization (reproduction)
fish roe
spawning
osmolality
egg
motility
gamete
teleost
methodology
hatchery
cavity
experiment
seminal plasma
in vitro fertilization
sperm motility

Keywords

  • Artificial reproduction
  • Common carp
  • In vitro fertilisation
  • Induced spawning

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Müller, T., Horváth, L., Szabó, T., Ittzés, I., Bognár, A., Faidt, P., ... Kucska, B. (2018). Novel method for induced propagation of fish: Sperm injection in oviducts and ovary/ovarian lavage with sperm. Aquaculture, 482, 124-129. https://doi.org/10.1016/j.aquaculture.2017.09.025

Novel method for induced propagation of fish : Sperm injection in oviducts and ovary/ovarian lavage with sperm. / Müller, T.; Horváth, L.; Szabó, T.; Ittzés, I.; Bognár, A.; Faidt, P.; Ittzés, ; Urbányi, B.; Kucska, B.

In: Aquaculture, Vol. 482, 01.01.2018, p. 124-129.

Research output: Contribution to journalArticle

Müller, T, Horváth, L, Szabó, T, Ittzés, I, Bognár, A, Faidt, P, Ittzés, , Urbányi, B & Kucska, B 2018, 'Novel method for induced propagation of fish: Sperm injection in oviducts and ovary/ovarian lavage with sperm', Aquaculture, vol. 482, pp. 124-129. https://doi.org/10.1016/j.aquaculture.2017.09.025
Müller, T. ; Horváth, L. ; Szabó, T. ; Ittzés, I. ; Bognár, A. ; Faidt, P. ; Ittzés, ; Urbányi, B. ; Kucska, B. / Novel method for induced propagation of fish : Sperm injection in oviducts and ovary/ovarian lavage with sperm. In: Aquaculture. 2018 ; Vol. 482. pp. 124-129.
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