Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer

Zita Olah, Ashley I. Bush, David Aleksza, Bence Galik, Eszter Ivitz, Lilla Macsai, Z. Janka, Zoltan Karman, Janos Kalman, Zsolt Datki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid rotifer model to use in high-throughput viability and non-invasive assays. In order to identify our species Philodina acuticornis odiosa (PA), 18 S rDNA-based phylogenetic analysis was carried out and their species-specific morphological markers identified. To execute the rotifer-based experiments, we developed an oil-covered water-drop methodology adapted from human in vitro fertilization techniques. This enables toxicological observations of individual one-housed rotifers in a closed and controllable micro-environment for up to several weeks. Hydrogen peroxide (H2O2) and sodium azide (NaN3) exposures were used as well-understood toxins. The toxicity and survival lifespan (TSL), the bright light disturbance (BLD) the mastax contraction frequency (MCF) and the cellular reduction capacity (CRC), indices were recorded. These newly developed assays were used to test the effects of lethal and sublethal doses of the toxins. The results showed the expected dose-dependent decrease in indices. These four different assays can either be used independently or as an integrated system for studying rotifers. These new indices render the PA invertebrate rotifer model a quantitative system for measuring viability, toxicity and lifespan (with TSL), systemic reaction capacity (with BLD), organic functionality (with MCF) and reductive capability of rotifers (with CRC), in vivo. This novel multi-level system is a reliable, sensitive and replicable screening tool with potential application in pharmaceutical science.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalEcotoxicology and Environmental Safety
Volume144
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Sodium Azide
Toxicology
Assays
Throughput
Toxicity
Light
Survival
Invertebrates
Fertilization in Vitro
Ribosomal DNA
Hydrogen Peroxide
Oils
Water
Research
Hydrogen peroxide
Pharmaceutical Preparations
Drug products
Screening
Aging of materials
Sodium

Keywords

  • 18 S rDNA
  • Bdelloid
  • Culturing
  • High throughput assay
  • Mastax
  • Oil-drop method
  • Oxidative stress
  • Pharmacological model
  • Philodina acuticornis odiosa
  • Rotifer

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer. / Olah, Zita; Bush, Ashley I.; Aleksza, David; Galik, Bence; Ivitz, Eszter; Macsai, Lilla; Janka, Z.; Karman, Zoltan; Kalman, Janos; Datki, Zsolt.

In: Ecotoxicology and Environmental Safety, Vol. 144, 01.10.2017, p. 115-122.

Research output: Contribution to journalArticle

Olah, Zita ; Bush, Ashley I. ; Aleksza, David ; Galik, Bence ; Ivitz, Eszter ; Macsai, Lilla ; Janka, Z. ; Karman, Zoltan ; Kalman, Janos ; Datki, Zsolt. / Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer. In: Ecotoxicology and Environmental Safety. 2017 ; Vol. 144. pp. 115-122.
@article{928178e11cd14f9f81b20b553c8ceb7d,
title = "Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer",
abstract = "Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid rotifer model to use in high-throughput viability and non-invasive assays. In order to identify our species Philodina acuticornis odiosa (PA), 18 S rDNA-based phylogenetic analysis was carried out and their species-specific morphological markers identified. To execute the rotifer-based experiments, we developed an oil-covered water-drop methodology adapted from human in vitro fertilization techniques. This enables toxicological observations of individual one-housed rotifers in a closed and controllable micro-environment for up to several weeks. Hydrogen peroxide (H2O2) and sodium azide (NaN3) exposures were used as well-understood toxins. The toxicity and survival lifespan (TSL), the bright light disturbance (BLD) the mastax contraction frequency (MCF) and the cellular reduction capacity (CRC), indices were recorded. These newly developed assays were used to test the effects of lethal and sublethal doses of the toxins. The results showed the expected dose-dependent decrease in indices. These four different assays can either be used independently or as an integrated system for studying rotifers. These new indices render the PA invertebrate rotifer model a quantitative system for measuring viability, toxicity and lifespan (with TSL), systemic reaction capacity (with BLD), organic functionality (with MCF) and reductive capability of rotifers (with CRC), in vivo. This novel multi-level system is a reliable, sensitive and replicable screening tool with potential application in pharmaceutical science.",
keywords = "18 S rDNA, Bdelloid, Culturing, High throughput assay, Mastax, Oil-drop method, Oxidative stress, Pharmacological model, Philodina acuticornis odiosa, Rotifer",
author = "Zita Olah and Bush, {Ashley I.} and David Aleksza and Bence Galik and Eszter Ivitz and Lilla Macsai and Z. Janka and Zoltan Karman and Janos Kalman and Zsolt Datki",
year = "2017",
month = "10",
day = "1",
doi = "10.1016/j.ecoenv.2017.06.005",
language = "English",
volume = "144",
pages = "115--122",
journal = "Ecotoxicology and Environmental Safety",
issn = "0147-6513",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer

AU - Olah, Zita

AU - Bush, Ashley I.

AU - Aleksza, David

AU - Galik, Bence

AU - Ivitz, Eszter

AU - Macsai, Lilla

AU - Janka, Z.

AU - Karman, Zoltan

AU - Kalman, Janos

AU - Datki, Zsolt

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid rotifer model to use in high-throughput viability and non-invasive assays. In order to identify our species Philodina acuticornis odiosa (PA), 18 S rDNA-based phylogenetic analysis was carried out and their species-specific morphological markers identified. To execute the rotifer-based experiments, we developed an oil-covered water-drop methodology adapted from human in vitro fertilization techniques. This enables toxicological observations of individual one-housed rotifers in a closed and controllable micro-environment for up to several weeks. Hydrogen peroxide (H2O2) and sodium azide (NaN3) exposures were used as well-understood toxins. The toxicity and survival lifespan (TSL), the bright light disturbance (BLD) the mastax contraction frequency (MCF) and the cellular reduction capacity (CRC), indices were recorded. These newly developed assays were used to test the effects of lethal and sublethal doses of the toxins. The results showed the expected dose-dependent decrease in indices. These four different assays can either be used independently or as an integrated system for studying rotifers. These new indices render the PA invertebrate rotifer model a quantitative system for measuring viability, toxicity and lifespan (with TSL), systemic reaction capacity (with BLD), organic functionality (with MCF) and reductive capability of rotifers (with CRC), in vivo. This novel multi-level system is a reliable, sensitive and replicable screening tool with potential application in pharmaceutical science.

AB - Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid rotifer model to use in high-throughput viability and non-invasive assays. In order to identify our species Philodina acuticornis odiosa (PA), 18 S rDNA-based phylogenetic analysis was carried out and their species-specific morphological markers identified. To execute the rotifer-based experiments, we developed an oil-covered water-drop methodology adapted from human in vitro fertilization techniques. This enables toxicological observations of individual one-housed rotifers in a closed and controllable micro-environment for up to several weeks. Hydrogen peroxide (H2O2) and sodium azide (NaN3) exposures were used as well-understood toxins. The toxicity and survival lifespan (TSL), the bright light disturbance (BLD) the mastax contraction frequency (MCF) and the cellular reduction capacity (CRC), indices were recorded. These newly developed assays were used to test the effects of lethal and sublethal doses of the toxins. The results showed the expected dose-dependent decrease in indices. These four different assays can either be used independently or as an integrated system for studying rotifers. These new indices render the PA invertebrate rotifer model a quantitative system for measuring viability, toxicity and lifespan (with TSL), systemic reaction capacity (with BLD), organic functionality (with MCF) and reductive capability of rotifers (with CRC), in vivo. This novel multi-level system is a reliable, sensitive and replicable screening tool with potential application in pharmaceutical science.

KW - 18 S rDNA

KW - Bdelloid

KW - Culturing

KW - High throughput assay

KW - Mastax

KW - Oil-drop method

KW - Oxidative stress

KW - Pharmacological model

KW - Philodina acuticornis odiosa

KW - Rotifer

UR - http://www.scopus.com/inward/record.url?scp=85020246355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020246355&partnerID=8YFLogxK

U2 - 10.1016/j.ecoenv.2017.06.005

DO - 10.1016/j.ecoenv.2017.06.005

M3 - Article

C2 - 28605645

AN - SCOPUS:85020246355

VL - 144

SP - 115

EP - 122

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

SN - 0147-6513

ER -