Novel Hydrolases from Thermophilic Filamentous Fungi for Enantiomerically and Enantiotopically Selective Biotransformations

Viktória Bódai, Réka Peredi, J. Bálint, G. Egri, L. Novák, G. Szakács, L. Poppe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Fourteen thermophilic filamentous fungi were cultivated on two shake flask media and the supernatants were assayed for lipase/carboxylesterase activities using olive-oil, p-nitrophenyl palmitate and p-nitrophenyl butyrate as substrates. The crude enzyme powders (acetone precipitated supernatants) were tested as biocatalysts in organic solvents. Kinetic resolution of racemic 1-phenylethanol (rac-1) and desymmetrisation of 2-acyloxypropan-1,3-diols (3a, b) by acetylation with vinyl acetate were chosen for testing the biocatalytic abilities of these preparations. The tested biocatalysts proved to be comparable to the commercially available enzymes with respect to the degree of enantiomer selectivity, whereas they exhibited a wider range of enantiotopic selectivity than the most common commercial enzymes.

Original languageEnglish
Pages (from-to)811-818
Number of pages8
JournalAdvanced Synthesis and Catalysis
Volume345
Issue number6-7
Publication statusPublished - Jun 2003

Fingerprint

Hydrolases
Fungi
Biocatalysts
Enzymes
Catalyst selectivity
Olive oil
Acetylation
Enantiomers
Lipases
Acetone
Organic solvents
Carboxylesterase
Palmitates
Powders
Kinetics
Lipase
Testing
Substrates
Biotransformation

Keywords

  • Asymmetric catalysis
  • Enzyme catalysis
  • Hydrolases
  • Kinetic resolution
  • Thermophilic filamentous fungi

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Organic Chemistry
  • Catalysis

Cite this

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author = "Vikt{\'o}ria B{\'o}dai and R{\'e}ka Peredi and J. B{\'a}lint and G. Egri and L. Nov{\'a}k and G. Szak{\'a}cs and L. Poppe",
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T1 - Novel Hydrolases from Thermophilic Filamentous Fungi for Enantiomerically and Enantiotopically Selective Biotransformations

AU - Bódai, Viktória

AU - Peredi, Réka

AU - Bálint, J.

AU - Egri, G.

AU - Novák, L.

AU - Szakács, G.

AU - Poppe, L.

PY - 2003/6

Y1 - 2003/6

N2 - Fourteen thermophilic filamentous fungi were cultivated on two shake flask media and the supernatants were assayed for lipase/carboxylesterase activities using olive-oil, p-nitrophenyl palmitate and p-nitrophenyl butyrate as substrates. The crude enzyme powders (acetone precipitated supernatants) were tested as biocatalysts in organic solvents. Kinetic resolution of racemic 1-phenylethanol (rac-1) and desymmetrisation of 2-acyloxypropan-1,3-diols (3a, b) by acetylation with vinyl acetate were chosen for testing the biocatalytic abilities of these preparations. The tested biocatalysts proved to be comparable to the commercially available enzymes with respect to the degree of enantiomer selectivity, whereas they exhibited a wider range of enantiotopic selectivity than the most common commercial enzymes.

AB - Fourteen thermophilic filamentous fungi were cultivated on two shake flask media and the supernatants were assayed for lipase/carboxylesterase activities using olive-oil, p-nitrophenyl palmitate and p-nitrophenyl butyrate as substrates. The crude enzyme powders (acetone precipitated supernatants) were tested as biocatalysts in organic solvents. Kinetic resolution of racemic 1-phenylethanol (rac-1) and desymmetrisation of 2-acyloxypropan-1,3-diols (3a, b) by acetylation with vinyl acetate were chosen for testing the biocatalytic abilities of these preparations. The tested biocatalysts proved to be comparable to the commercially available enzymes with respect to the degree of enantiomer selectivity, whereas they exhibited a wider range of enantiotopic selectivity than the most common commercial enzymes.

KW - Asymmetric catalysis

KW - Enzyme catalysis

KW - Hydrolases

KW - Kinetic resolution

KW - Thermophilic filamentous fungi

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