Novel genes in human asthma based on a mouse model of allergic airway inflammation and human investigations

Gergely Temesi, Viktor Virág, Éva Hadadi, Ildikó Ungvári, Lili E. Fodor, András Bikov, Adrienne Nagy, Gabriella Gálffy, Lilla Tamási, Ildikó Horváth, András Kiss, Gábor Hullám, András Gézsi, Péter Sárközy, Péter Antal, Edit Buzás, Csaba Szalai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: Based on a previous gene expression study in a mouse model of asthma, we selected 60 candidate genes and investigated their possible roles in human asthma. Methods: In these candidate genes, 90 SNPs were genotyped using MassARRAY technology from 311 asthmatic children and 360 healthy controls of the Hungarian (Caucasian) population. Moreover, gene expression levels were measured by RT PCR in the induced sputum of 13 asthmatics and 10 control individuals. t-tests, chi-square tests, and logistic regression were carried out in order to assess associations of SNP frequency and expression level with asthma. Permutation tests were performed to account for multiple hypothesis testing. Results: The frequency of 4 SNPs in 2 genes differed significantly between asthmatic and control subjects: SNPs rs2240572, rs2240571, rs3735222 in gene SCIN, and rs32588 in gene PPARGC1B. Carriers of the minor alleles had reduced risk of asthma with an odds ratio of 0.64 (0.51-0.80; P=7×10-5) in SCIN and 0.56 (0.42-0.76; P=1.2×10-4) in PPARGC1B. The expression levels of SCIN, PPARGC1B and ITLN1 genes were significantly lower in the sputum of asthmatics. Conclusions: Three potentially novel asthma-associated genes were identified based on mouse experiments and human studies.

Original languageEnglish
Pages (from-to)496-503
Number of pages8
JournalAllergy, Asthma and Immunology Research
Volume6
Issue number6
DOIs
Publication statusPublished - Jan 1 2014

Keywords

  • Animal
  • Asthma
  • Child
  • Disease model
  • Genetic association studies, gene expression
  • Sputum

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

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    Temesi, G., Virág, V., Hadadi, É., Ungvári, I., Fodor, L. E., Bikov, A., Nagy, A., Gálffy, G., Tamási, L., Horváth, I., Kiss, A., Hullám, G., Gézsi, A., Sárközy, P., Antal, P., Buzás, E., & Szalai, C. (2014). Novel genes in human asthma based on a mouse model of allergic airway inflammation and human investigations. Allergy, Asthma and Immunology Research, 6(6), 496-503. https://doi.org/10.4168/aair.2014.6.6.496