Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells

E. Csonka, I. Cserpan, K. Fodor, G. Hollo, R. Katona, J. Kereso, T. Praznovszky, B. Szakal, A. Telenius, G. deJong, A. Udvardy, G. Hadlaczky

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

An in vivo approach has been developed for generation of artificial chromosomes, based on the induction of intrinsic, large-scale amplification mechanisms of mammalian cells. Here, we describe the successful generation of prototype human satellite DNA-based artificial chromosomes via amplification-dependent de novo chromosome formations induced by integration of exogenous DNA sequences into the centromeric/rDNA regions of human acrocentric chromosomes. Subclones with mitotically stable de novo chromosomes were established, which allowed the initial characterization and purification of these artificial chromosomes. Because of the low complexity of their DNA content, they may serve as a useful tool to study the structure and function of higher eukaryotic chromosomes. Human satellite DNA-based artificial chromosomes containing amplified satellite DNA, rDNA, and exogenous DNA sequences were heterochromatic, however, they provided a suitable chromosomal environment for the expression of the integrated exogenous genetic material. We demonstrate that induced de novo chromosome formation is a reproducible and effective methodology in generating artificial chromosomes from predictable sequences of different mammalian species. Satellite DNA-based artificial chromosomes formed by induced large-scale amplifications on the short arm of human acrocentric chromosomes may become safe or low risk vectors in gene therapy.

Original languageEnglish
Pages (from-to)3207-3216
Number of pages10
JournalJournal of Cell Science
Volume113
Issue number18
Publication statusPublished - 2000

Fingerprint

Mammalian Artificial Chromosomes
Artificial Chromosomes
Satellite DNA
Chromosomes
Human Chromosomes
Ribosomal DNA
Genetic Therapy
DNA

Keywords

  • Human satellite DNA-based artificial chromosome
  • Large-scale amplification
  • RDNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Csonka, E., Cserpan, I., Fodor, K., Hollo, G., Katona, R., Kereso, J., ... Hadlaczky, G. (2000). Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells. Journal of Cell Science, 113(18), 3207-3216.

Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells. / Csonka, E.; Cserpan, I.; Fodor, K.; Hollo, G.; Katona, R.; Kereso, J.; Praznovszky, T.; Szakal, B.; Telenius, A.; deJong, G.; Udvardy, A.; Hadlaczky, G.

In: Journal of Cell Science, Vol. 113, No. 18, 2000, p. 3207-3216.

Research output: Contribution to journalArticle

Csonka, E, Cserpan, I, Fodor, K, Hollo, G, Katona, R, Kereso, J, Praznovszky, T, Szakal, B, Telenius, A, deJong, G, Udvardy, A & Hadlaczky, G 2000, 'Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells', Journal of Cell Science, vol. 113, no. 18, pp. 3207-3216.
Csonka E, Cserpan I, Fodor K, Hollo G, Katona R, Kereso J et al. Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells. Journal of Cell Science. 2000;113(18):3207-3216.
Csonka, E. ; Cserpan, I. ; Fodor, K. ; Hollo, G. ; Katona, R. ; Kereso, J. ; Praznovszky, T. ; Szakal, B. ; Telenius, A. ; deJong, G. ; Udvardy, A. ; Hadlaczky, G. / Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells. In: Journal of Cell Science. 2000 ; Vol. 113, No. 18. pp. 3207-3216.
@article{7c4060d1f8b8458c8f246c26abbc5b6e,
title = "Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells",
abstract = "An in vivo approach has been developed for generation of artificial chromosomes, based on the induction of intrinsic, large-scale amplification mechanisms of mammalian cells. Here, we describe the successful generation of prototype human satellite DNA-based artificial chromosomes via amplification-dependent de novo chromosome formations induced by integration of exogenous DNA sequences into the centromeric/rDNA regions of human acrocentric chromosomes. Subclones with mitotically stable de novo chromosomes were established, which allowed the initial characterization and purification of these artificial chromosomes. Because of the low complexity of their DNA content, they may serve as a useful tool to study the structure and function of higher eukaryotic chromosomes. Human satellite DNA-based artificial chromosomes containing amplified satellite DNA, rDNA, and exogenous DNA sequences were heterochromatic, however, they provided a suitable chromosomal environment for the expression of the integrated exogenous genetic material. We demonstrate that induced de novo chromosome formation is a reproducible and effective methodology in generating artificial chromosomes from predictable sequences of different mammalian species. Satellite DNA-based artificial chromosomes formed by induced large-scale amplifications on the short arm of human acrocentric chromosomes may become safe or low risk vectors in gene therapy.",
keywords = "Human satellite DNA-based artificial chromosome, Large-scale amplification, RDNA",
author = "E. Csonka and I. Cserpan and K. Fodor and G. Hollo and R. Katona and J. Kereso and T. Praznovszky and B. Szakal and A. Telenius and G. deJong and A. Udvardy and G. Hadlaczky",
year = "2000",
language = "English",
volume = "113",
pages = "3207--3216",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "18",

}

TY - JOUR

T1 - Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells

AU - Csonka, E.

AU - Cserpan, I.

AU - Fodor, K.

AU - Hollo, G.

AU - Katona, R.

AU - Kereso, J.

AU - Praznovszky, T.

AU - Szakal, B.

AU - Telenius, A.

AU - deJong, G.

AU - Udvardy, A.

AU - Hadlaczky, G.

PY - 2000

Y1 - 2000

N2 - An in vivo approach has been developed for generation of artificial chromosomes, based on the induction of intrinsic, large-scale amplification mechanisms of mammalian cells. Here, we describe the successful generation of prototype human satellite DNA-based artificial chromosomes via amplification-dependent de novo chromosome formations induced by integration of exogenous DNA sequences into the centromeric/rDNA regions of human acrocentric chromosomes. Subclones with mitotically stable de novo chromosomes were established, which allowed the initial characterization and purification of these artificial chromosomes. Because of the low complexity of their DNA content, they may serve as a useful tool to study the structure and function of higher eukaryotic chromosomes. Human satellite DNA-based artificial chromosomes containing amplified satellite DNA, rDNA, and exogenous DNA sequences were heterochromatic, however, they provided a suitable chromosomal environment for the expression of the integrated exogenous genetic material. We demonstrate that induced de novo chromosome formation is a reproducible and effective methodology in generating artificial chromosomes from predictable sequences of different mammalian species. Satellite DNA-based artificial chromosomes formed by induced large-scale amplifications on the short arm of human acrocentric chromosomes may become safe or low risk vectors in gene therapy.

AB - An in vivo approach has been developed for generation of artificial chromosomes, based on the induction of intrinsic, large-scale amplification mechanisms of mammalian cells. Here, we describe the successful generation of prototype human satellite DNA-based artificial chromosomes via amplification-dependent de novo chromosome formations induced by integration of exogenous DNA sequences into the centromeric/rDNA regions of human acrocentric chromosomes. Subclones with mitotically stable de novo chromosomes were established, which allowed the initial characterization and purification of these artificial chromosomes. Because of the low complexity of their DNA content, they may serve as a useful tool to study the structure and function of higher eukaryotic chromosomes. Human satellite DNA-based artificial chromosomes containing amplified satellite DNA, rDNA, and exogenous DNA sequences were heterochromatic, however, they provided a suitable chromosomal environment for the expression of the integrated exogenous genetic material. We demonstrate that induced de novo chromosome formation is a reproducible and effective methodology in generating artificial chromosomes from predictable sequences of different mammalian species. Satellite DNA-based artificial chromosomes formed by induced large-scale amplifications on the short arm of human acrocentric chromosomes may become safe or low risk vectors in gene therapy.

KW - Human satellite DNA-based artificial chromosome

KW - Large-scale amplification

KW - RDNA

UR - http://www.scopus.com/inward/record.url?scp=0033785931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033785931&partnerID=8YFLogxK

M3 - Article

VL - 113

SP - 3207

EP - 3216

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 18

ER -