Novel complexes of cyclin-dependent kinases and a cyclin-like protein from Arabidopsis thaliana with a function unrelated to cell division

R. M. Barrôco, L. De Veylder, Z. Magyar, G. Engler, D. Inzé, V. Mironov

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Although the majority of cyclin-dependent kinases (CDKs) play a key role in cell cycle progression, recent evidence has shown that CDKs are also implicated in transcription regulation. Here, we describe two Arabidopsis CDKs designated Arath;CDKC;1 and Arath;CDKC;2. These CDKs share a PITAIRE signature in the cyclin-binding domain and the structural characteristics of mammalian CDK9. Yeast two-hybrid screens and immunoprecipitation assays identified CDKC-interacting proteins with homology to the animal cyclin T/cyclin K group. We suggest that these Arabidopsis CDKCs may be part of a kinase complex similar to the animal positive transcription elongation factor b, whose activity is essential for transcription control. Expression studies showed that Arath; CDKC transcripts are mainly confined to epidermal tissues and are most abundant in flower tissues. No expression was detected in actively dividing Arabidopsis tissues, suggesting a role for the CDKC proteins in differentiated cells.

Original languageEnglish
Pages (from-to)401-412
Number of pages12
JournalCellular and Molecular Life Sciences
Volume60
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Keywords

  • Arabidopsis thaliana
  • Cell cycle
  • Cyclin T
  • Cyclin-dependent kinase (CDK)
  • Transcription control
  • Two-hybrid interaction

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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