Novel bioreactor platform for scalable cardiomyogenic differentiation from pluripotent stem cell-derived embryoid bodies

Sasitorn Rungarunlert, Joao N. Ferreira, Andras Dinnyes

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Generation of cardiomyocytes from pluripotent stem cells (PSCs) is a common and valuable approach to produce large amount of cells for various applications, including assays and models for drug development, cell-based therapies, and tissue engineering. All these applications would benefit from a reliable bioreactorbased methodology to consistently generate homogenous PSC-derived embryoid bodies (EBs) at a large scale, which can further undergo cardiomyogenic differentiation. The goal of this chapter is to describe a scalable method to consistently generate large amount of homogeneous and synchronized EBs from PSCs. This method utilizes a slow-turning lateral vessel bioreactor to direct the EB formation and their subsequent cardiomyogenic lineage differentiation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages169-179
Number of pages11
Volume1502
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1502
ISSN (Print)10643745

Fingerprint

Embryoid Bodies
Pluripotent Stem Cells
Bioreactors
Tissue Engineering
Cell- and Tissue-Based Therapy
Cardiac Myocytes
Pharmaceutical Preparations

Keywords

  • Bioreactor
  • Cardiomyocyte
  • Embryoid body
  • Pluripotent stem cells
  • Slow turning lateral vessel

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Rungarunlert, S., Ferreira, J. N., & Dinnyes, A. (2016). Novel bioreactor platform for scalable cardiomyogenic differentiation from pluripotent stem cell-derived embryoid bodies. In Methods in Molecular Biology (Vol. 1502, pp. 169-179). (Methods in Molecular Biology; Vol. 1502). Humana Press Inc.. https://doi.org/10.1007/7651_2016_341

Novel bioreactor platform for scalable cardiomyogenic differentiation from pluripotent stem cell-derived embryoid bodies. / Rungarunlert, Sasitorn; Ferreira, Joao N.; Dinnyes, Andras.

Methods in Molecular Biology. Vol. 1502 Humana Press Inc., 2016. p. 169-179 (Methods in Molecular Biology; Vol. 1502).

Research output: Chapter in Book/Report/Conference proceedingChapter

Rungarunlert, S, Ferreira, JN & Dinnyes, A 2016, Novel bioreactor platform for scalable cardiomyogenic differentiation from pluripotent stem cell-derived embryoid bodies. in Methods in Molecular Biology. vol. 1502, Methods in Molecular Biology, vol. 1502, Humana Press Inc., pp. 169-179. https://doi.org/10.1007/7651_2016_341
Rungarunlert S, Ferreira JN, Dinnyes A. Novel bioreactor platform for scalable cardiomyogenic differentiation from pluripotent stem cell-derived embryoid bodies. In Methods in Molecular Biology. Vol. 1502. Humana Press Inc. 2016. p. 169-179. (Methods in Molecular Biology). https://doi.org/10.1007/7651_2016_341
Rungarunlert, Sasitorn ; Ferreira, Joao N. ; Dinnyes, Andras. / Novel bioreactor platform for scalable cardiomyogenic differentiation from pluripotent stem cell-derived embryoid bodies. Methods in Molecular Biology. Vol. 1502 Humana Press Inc., 2016. pp. 169-179 (Methods in Molecular Biology).
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