Novel aspects of the electrophysiology of mitochondrial porin

György Báthori, Ildikó Szabó, Ibolya Schmehl, Francesco Tombola, Vito De Pinto, Mario Zoratti

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The recent findings that mitochondrial porin, VDAC, participates in supramolecular complexes and is present in the plasmamembrane need to be reconciled with its biophysical properties. We report here that VDAC often displays previously unobserved or unappreciated behaviors. Reconstituted VDAC can: a) exhibit fast gating when in any of many conductance substates; b) close completely, although briefly, on its own; c) close for long periods, in the presence of Konig's polyanion; d) take several milliseconds to re-open when an applied transmembrane potential is switched off; e) be desensitized by prolonged exposure to high voltages, so that it will not re-open to the full conductance state upon subsequent return to zero voltage; f) display polarity dependent voltage-induced closure. These behaviors are especially noticeable when the observations are conducted on a single reincorporated channel, suggesting that interactions between copies of VDAC may play a role in determining its electrophysiological properties. Any model of VDAC's structure, gating and function should take these observations into account.

Original languageEnglish
Pages (from-to)258-263
Number of pages6
JournalBiochemical and biophysical research communications
Volume243
Issue number1
DOIs
Publication statusPublished - Feb 4 1998

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Keywords

  • Bilayer
  • Gating kinetics
  • Mitochondrial porin
  • Substates

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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