Novel anti-cholesterol monoclonal immunoglobulin G antibodies as probes and potential modulators of membrane raft-dependent immune functions

Adrienn Bíró, L. Cervenák, Andrea Balogh, A. Lőrincz, K. Uray, A. Horváth, László Romics, J. Matkó, G. Füst, Glória László

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Natural autoantibodies against cholesterol are present in the sera of all healthy individuals; their function, production, and regulation, however, are still unclear. Here, we managed to produce two monoclonal anti-cholesterol antibodies (ACHAs) by immunizing mice with cholesterolrich liposomes. The new ACHAs were specific to cholesterol and to some structurally closely related 3β-hydroxyl sterols, and they reacted with human lipoproteins VLDL, LDL, and HDL. They bound, usually with low avidity, to live human or murine lymphocyte and monocyte-macrophage cell lines, which was enhanced substantially by a moderate papain digestion of the cell surface, removing some protruding extracellular protein domains. Cell-bound ACHAs strongly colocalized with markers of cholesterol-rich lipid rafts and caveolae at the cell surface and intracellularly with markers of the endoplasmic reticulum and Golgi complex. These data suggest that these IgG ACHAs may serve as probes of clustered cholesterol (e.g., different lipid rafts) in live cells and thus may also have immunomodulatory potential.

Original languageEnglish
Pages (from-to)19-29
Number of pages11
JournalJournal of Lipid Research
Volume48
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Membrane Potentials
Modulators
Immunoglobulin G
Cholesterol
Membranes
Antibodies
Anti-Idiotypic Antibodies
Lipids
Caveolae
Papain
Lymphocytes
VLDL Lipoproteins
Macrophages
Golgi Apparatus
Sterols
HDL Lipoproteins
LDL Lipoproteins
Liposomes
Endoplasmic Reticulum
Hydroxyl Radical

Keywords

  • Caveolae
  • Lipid rafts
  • Lipoproteins
  • Lymphocytes
  • Membrane cholesterol
  • Monocyte-macrophage cells

ASJC Scopus subject areas

  • Endocrinology

Cite this

Novel anti-cholesterol monoclonal immunoglobulin G antibodies as probes and potential modulators of membrane raft-dependent immune functions. / Bíró, Adrienn; Cervenák, L.; Balogh, Andrea; Lőrincz, A.; Uray, K.; Horváth, A.; Romics, László; Matkó, J.; Füst, G.; László, Glória.

In: Journal of Lipid Research, Vol. 48, No. 1, 01.2007, p. 19-29.

Research output: Contribution to journalArticle

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