Novel and sensitive qPCR assays for the detection and identification of aspergillosis causing species

Melinda Paholcsek, E. Leiter, Arnold Markovics, Sándor Biró

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Despite concerted efforts, diagnosis of aspergillosis is still a great challenge to clinical microbiology laboratories. Along with the requirement for high sensitivity and specificity, species-specific identification is important. We developed rapid, sensitive and species-specific qPCR assays using the TaqMan technology for the detection and identification of Aspergillus fumigatus and Aspergillus terreus. The assays were designed to target orthologs of the Streptomyces factor C gene that are only found in a few species of filamentous fungi. Fungi acquired this gene through horizontal gene transfer and divergence of the gene allows identification of species. The assays have potential as a molecular diagnosis tool for the early detection of fungal infection caused by Aspergillus fumigatus and Aspergillus terreus, which merits future diagnostic studies. The assays were sensitive enough to detect a few genomic equivalents in blood samples.

Original languageEnglish
Pages (from-to)273-284
Number of pages12
JournalActa Microbiologica et Immunologica Hungarica
Volume61
Issue number3
DOIs
Publication statusPublished - Sep 1 2014

Fingerprint

Aspergillosis
Aspergillus fumigatus
Aspergillus
Fungi
Genes
Horizontal Gene Transfer
Mycoses
Microbiology
Technology
Sensitivity and Specificity

Keywords

  • aspergillosis
  • Aspergillus fumigatus
  • Aspergillus terreus
  • qPCR
  • Streptomyces factor C gene

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Novel and sensitive qPCR assays for the detection and identification of aspergillosis causing species. / Paholcsek, Melinda; Leiter, E.; Markovics, Arnold; Biró, Sándor.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 61, No. 3, 01.09.2014, p. 273-284.

Research output: Contribution to journalArticle

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