Normothermic Ex Vivo Kidney Perfusion Improves Early DCD Graft Function Compared with Hypothermic Machine Perfusion and Static Cold Storage

Peter Urbanellis, Matyas Hamar, J. Moritz Kaths, Dagmar Kollmann, Ivan Linares, Laura Mazilescu, Sujani Ganesh, Aryn Wiebe, Paul M. Yip, Rohan John, Ana Konvalinka, Istvan Mucsi, Anand Ghanekar, Darius J. Bagli, Lisa A. Robinson, Markus Selzner

Research output: Contribution to journalArticle

Abstract

Background. Better preservation strategies for the storage of donation after circulatory death grafts are essential to improve graft function and to increase the kidney donor pool. We compared continuous normothermic ex vivo kidney perfusion (NEVKP) with hypothermic anoxic machine perfusion (HAMP) and static cold storage (SCS) in a porcine kidney autotransplantation model. Methods. Porcine kidneys were exposed to 30 minutes of warm ischemia and then reimplanted following either 16 hours of either SCS, HAMP (LifePort 1.0), or NEVKP before autotransplantation (n = 5 per group). The contralateral kidney was removed. Animals were followed for 8 days. Results. Grafts preserved by NEVKP demonstrated improved function with more rapid recovery compared with HAMP and SCS (mean peak serum creatinine: 3.66 ± 1.33 mg/dL [postoperative d 1 [(POD1)], 8.82 ± 3.17 mg/dL [POD2], and 12.90 ± 2.19 mg/dL [POD3], respectively). The NEVKP group demonstrated significantly increased creatinine clearance calculated on POD3 (63.6 ± 19.0 mL/min) compared with HAMP (13.5 ± 10.3 mL/min, P = 0.001) and SCS (4.0 ± 2.6 mL/min, P = 0.001). Histopathologic injury scores on POD8 were lower in both perfused groups (NEVKP and HAMP, score: 1-1.5) compared with SCS (score: 1-3, P = 0.3), without reaching statistical significance. Conclusions. NEVKP storage significantly improved early kidney function compared with both cold preservation strategies, although HAMP also demonstrates improvement over SCS. NEVKP may represent a novel, superior preservation option for donation after circulatory death renal grafts compared with conventional hypothermic methods.

Original languageEnglish
Pages (from-to)947-955
Number of pages9
JournalTransplantation
DOIs
Publication statusAccepted/In press - 2020

ASJC Scopus subject areas

  • Transplantation

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    Urbanellis, P., Hamar, M., Kaths, J. M., Kollmann, D., Linares, I., Mazilescu, L., Ganesh, S., Wiebe, A., Yip, P. M., John, R., Konvalinka, A., Mucsi, I., Ghanekar, A., Bagli, D. J., Robinson, L. A., & Selzner, M. (Accepted/In press). Normothermic Ex Vivo Kidney Perfusion Improves Early DCD Graft Function Compared with Hypothermic Machine Perfusion and Static Cold Storage. Transplantation, 947-955. https://doi.org/10.1097/TP.0000000000003066