Normal skeletal development of mice lacking matrilin 1

Redundant function of matrilins in cartilage?

Attila Aszódi, John F. Bateman, Emilio Hirsch, Mária Baranyi, Ernst B. Hunziker, Nik Hauser, Z. Bösze, Reinhard Fässler

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Matrilin 1, or cartilage matrix protein, is a member of a novel family of extracellular matrix proteins. To date, four members of the family have been identified, but their biological role is unknown. Matrilin 1 and matrilin 3 are expressed in cartilage, while matrilin 2 and matrilin 4 are present in many tissues. Here we describe the generation and analysis of mice carrying a null mutation in the Crtm gene encoding matrilin 1. Anatomical and histological studies demonstrated normal development of homozygous mutant mice. Northern blot and biochemical analyses show no compensatory up- regulation of matrilin 2 or 3 in the cartilage of knockout mice. Although matrilin 1 interacts with the collagen II and aggrecan networks of cartilage, suggesting that it may play a role in cartilage tissue organization, studies of collagen extractability indicated that collagen fibril maturation and covalent cross-linking were unaffected by the absence of matrilin 1. Ultrastructural analysis did not reveal any abnormalities of matrix organization. These data suggest that matrilin 1 is not critically required for cartilage structure and function and that matrilin 1 and matrilin 3 may have functionally redundant roles.

Original languageEnglish
Pages (from-to)7841-7845
Number of pages5
JournalMolecular and Cellular Biology
Volume19
Issue number11
Publication statusPublished - Nov 1999

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Matrilin Proteins
Cartilage
Collagen
Aggrecans
Extracellular Matrix Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Aszódi, A., Bateman, J. F., Hirsch, E., Baranyi, M., Hunziker, E. B., Hauser, N., ... Fässler, R. (1999). Normal skeletal development of mice lacking matrilin 1: Redundant function of matrilins in cartilage? Molecular and Cellular Biology, 19(11), 7841-7845.

Normal skeletal development of mice lacking matrilin 1 : Redundant function of matrilins in cartilage? / Aszódi, Attila; Bateman, John F.; Hirsch, Emilio; Baranyi, Mária; Hunziker, Ernst B.; Hauser, Nik; Bösze, Z.; Fässler, Reinhard.

In: Molecular and Cellular Biology, Vol. 19, No. 11, 11.1999, p. 7841-7845.

Research output: Contribution to journalArticle

Aszódi, A, Bateman, JF, Hirsch, E, Baranyi, M, Hunziker, EB, Hauser, N, Bösze, Z & Fässler, R 1999, 'Normal skeletal development of mice lacking matrilin 1: Redundant function of matrilins in cartilage?', Molecular and Cellular Biology, vol. 19, no. 11, pp. 7841-7845.
Aszódi A, Bateman JF, Hirsch E, Baranyi M, Hunziker EB, Hauser N et al. Normal skeletal development of mice lacking matrilin 1: Redundant function of matrilins in cartilage? Molecular and Cellular Biology. 1999 Nov;19(11):7841-7845.
Aszódi, Attila ; Bateman, John F. ; Hirsch, Emilio ; Baranyi, Mária ; Hunziker, Ernst B. ; Hauser, Nik ; Bösze, Z. ; Fässler, Reinhard. / Normal skeletal development of mice lacking matrilin 1 : Redundant function of matrilins in cartilage?. In: Molecular and Cellular Biology. 1999 ; Vol. 19, No. 11. pp. 7841-7845.
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