Nonribosomal peptide synthetase genes in the genome of Fusarium graminearum, causative agent of wheat head blight

J. Varga, S. Kocsubé, Beáta Tóth, Á Mesterházy

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fungal nonribosomal peptide synthetases (NRPSs) are responsible for the biosynthesis of numerous metabolites which serve as virulence factors in several plant-pathogen interactions. The aim of our work was to investigate the diversity of these genes in a Fusarium graminearum sequence database using bioinformatic techniques. Our search identified 15 NRPS sequences, among which two were found to be closely related to peptide synthetases of various fungi taking part in ferrichrome biosynthesis. Another peptide synthetase gene was similar to that identified in Aspergillus oryzae which is possibly responsible for the biosynthesis of fusarinine, an extracellular iron-chelating siderophore. To our knowledge, this is the first report on the identification of a putative NRPS gene possibly responsible for the biosynthesis of fusarinine-type siderophores. The other NRPSs were found to be related to peptide synthetases taking part in the biosynthesis of various peptides in other fungi. Transcription factors carrying ankyrin repeats were observed in the vicinity of four of the identified peptide synthetase genes. Additionally, NRPS related genes similar to putative long-chain fatty acid CoA ligases, acyl CoA ligases, ABC transport proteins, a highly conserved putative transmembrane protein of Aspergillus nidulans, and α-aminoadipate reductases have also been identified. Further studies are in progress to clarify the role of some of the identified NRPS genes in plant pathogenesis.

Original languageEnglish
Pages (from-to)375-388
Number of pages14
JournalActa Biologica Hungarica
Volume56
Issue number3-4
DOIs
Publication statusPublished - 2005

Fingerprint

Peptide Synthases
head blight
Fusarium graminearum
Fusarium
ligases
peptide
Triticum
genome
Genes
wheat
Genome
peptides
gene
Biosynthesis
genes
biosynthesis
Siderophores
Aspergillus
siderophore
siderophores

Keywords

  • Fusarium graminearum
  • Genomics
  • Gibberella zeae
  • Nonribosomal peptide synthetase
  • Phylogenetics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Nonribosomal peptide synthetase genes in the genome of Fusarium graminearum, causative agent of wheat head blight. / Varga, J.; Kocsubé, S.; Tóth, Beáta; Mesterházy, Á.

In: Acta Biologica Hungarica, Vol. 56, No. 3-4, 2005, p. 375-388.

Research output: Contribution to journalArticle

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