Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients

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34 Citations (Scopus)

Abstract

A noninvasive DNA sampling method has been implemented collecting buccal mucosa cells by cotton wool swabs. An amount of 0.2-2 μg DNA per patient was obtained after the phenol-extraction procedure and 0.2-2 ng DNA template was sufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopamine receptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separation by laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drug-dependent subjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variants beside the common 4-repeat DRD4 allele.

Original languageEnglish
Pages (from-to)793-797
Number of pages5
JournalCurrent Medicinal Chemistry
Volume9
Issue number8
Publication statusPublished - 2002

Fingerprint

Dopamine Receptors
DNA
Dopamine D4 Receptors
Polymerase Chain Reaction
Wool
Mouth Mucosa
Heterozygote
Phenol
Cotton
Amplification
Lasers
Genes
Alleles
Sampling
Pharmaceutical Preparations

Keywords

  • Buccal swab
  • Dopamine D4 receptor (DRD4)
  • Genotyping
  • Noninvasive DNA sampling
  • Substance-dependence

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

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title = "Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients",
abstract = "A noninvasive DNA sampling method has been implemented collecting buccal mucosa cells by cotton wool swabs. An amount of 0.2-2 μg DNA per patient was obtained after the phenol-extraction procedure and 0.2-2 ng DNA template was sufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopamine receptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separation by laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drug-dependent subjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variants beside the common 4-repeat DRD4 allele.",
keywords = "Buccal swab, Dopamine D4 receptor (DRD4), Genotyping, Noninvasive DNA sampling, Substance-dependence",
author = "K. Bo{\'o}r and Z. R{\'o}nai and Z. Nemoda and P. Gaszner and M. Sasv{\'a}ri and A. Guttman and H. Kal{\'a}sz",
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T1 - Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients

AU - Boór, K.

AU - Rónai, Z.

AU - Nemoda, Z.

AU - Gaszner, P.

AU - Sasvári, M.

AU - Guttman, A.

AU - Kalász, H.

PY - 2002

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