Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients

Research output: Contribution to journalReview article

36 Citations (Scopus)

Abstract

A noninvasive DNA sampling method has been implemented collecting buccal mucosa cells by cotton wool swabs. An amount of 0.2-2 μg DNA per patient was obtained after the phenol-extraction procedure and 0.2-2 ng DNA template was sufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopamine receptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separation by laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drug-dependent subjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variants beside the common 4-repeat DRD4 allele.

Original languageEnglish
Pages (from-to)793-797
Number of pages5
JournalCurrent medicinal chemistry
Volume9
Issue number8
DOIs
Publication statusPublished - Jan 1 2002

Keywords

  • Buccal swab
  • Dopamine D4 receptor (DRD4)
  • Genotyping
  • Noninvasive DNA sampling
  • Substance-dependence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients'. Together they form a unique fingerprint.

  • Cite this