Non-TIR-NBS-LRR resistance gene analogs in apricot (Prunus armeniaca L.)

Á Gutermuth, Zsuzsanna György, A. Hegedüs, A. Pedryc

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Genes encoding for proteins with nucleotide-binding site and leucine-rich repeat motifs (NBS-LRR) have been suggested to play a general role in plant defence mechanism. In Prunus species, many TIR (Toll / Interleukin-1 Receptor), and only very few non-TIR sequences were identified, which was explained either by the unequal distribution of TIR/non-TIR sequences in the Prunus genome or by the incapability of primers in the amplification of non-TIR RGAs. The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosaceaa sequences. A significantly higher ratio (91%) of non-TIR sequences were arranged in multi-genera clades than that of (57%) the TIR groups confirming that non-TIR sequences might be of more ancient origin than TIR sequences.

Original languageEnglish
Pages (from-to)171-181
Number of pages11
JournalActa Biologica Hungarica
Volume62
Issue number2
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

Interleukin-1 Receptors
Leucine
amplification
Nucleotides
Genes
Binding Sites
polymerase chain reaction
gene
clone
plant defense
defense mechanism
genome
phylogenetics
Amplification
protein
Polymerase chain reaction
Prunus
Prunus armeniaca
Clone Cells
Rosaceae

Keywords

  • Apricot
  • non-TIR-NBS-LRR
  • Prunus armeniaca L.
  • resistance gene analogs
  • RGA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Non-TIR-NBS-LRR resistance gene analogs in apricot (Prunus armeniaca L.). / Gutermuth, Á; György, Zsuzsanna; Hegedüs, A.; Pedryc, A.

In: Acta Biologica Hungarica, Vol. 62, No. 2, 01.06.2011, p. 171-181.

Research output: Contribution to journalArticle

@article{fd76dc2ea87c42e0b0fe8bd316a0e206,
title = "Non-TIR-NBS-LRR resistance gene analogs in apricot (Prunus armeniaca L.)",
abstract = "Genes encoding for proteins with nucleotide-binding site and leucine-rich repeat motifs (NBS-LRR) have been suggested to play a general role in plant defence mechanism. In Prunus species, many TIR (Toll / Interleukin-1 Receptor), and only very few non-TIR sequences were identified, which was explained either by the unequal distribution of TIR/non-TIR sequences in the Prunus genome or by the incapability of primers in the amplification of non-TIR RGAs. The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosaceaa sequences. A significantly higher ratio (91{\%}) of non-TIR sequences were arranged in multi-genera clades than that of (57{\%}) the TIR groups confirming that non-TIR sequences might be of more ancient origin than TIR sequences.",
keywords = "Apricot, non-TIR-NBS-LRR, Prunus armeniaca L., resistance gene analogs, RGA",
author = "{\'A} Gutermuth and Zsuzsanna Gy{\"o}rgy and A. Heged{\"u}s and A. Pedryc",
year = "2011",
month = "6",
day = "1",
doi = "10.1556/ABiol.62.2011.2.7",
language = "English",
volume = "62",
pages = "171--181",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Non-TIR-NBS-LRR resistance gene analogs in apricot (Prunus armeniaca L.)

AU - Gutermuth, Á

AU - György, Zsuzsanna

AU - Hegedüs, A.

AU - Pedryc, A.

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Genes encoding for proteins with nucleotide-binding site and leucine-rich repeat motifs (NBS-LRR) have been suggested to play a general role in plant defence mechanism. In Prunus species, many TIR (Toll / Interleukin-1 Receptor), and only very few non-TIR sequences were identified, which was explained either by the unequal distribution of TIR/non-TIR sequences in the Prunus genome or by the incapability of primers in the amplification of non-TIR RGAs. The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosaceaa sequences. A significantly higher ratio (91%) of non-TIR sequences were arranged in multi-genera clades than that of (57%) the TIR groups confirming that non-TIR sequences might be of more ancient origin than TIR sequences.

AB - Genes encoding for proteins with nucleotide-binding site and leucine-rich repeat motifs (NBS-LRR) have been suggested to play a general role in plant defence mechanism. In Prunus species, many TIR (Toll / Interleukin-1 Receptor), and only very few non-TIR sequences were identified, which was explained either by the unequal distribution of TIR/non-TIR sequences in the Prunus genome or by the incapability of primers in the amplification of non-TIR RGAs. The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosaceaa sequences. A significantly higher ratio (91%) of non-TIR sequences were arranged in multi-genera clades than that of (57%) the TIR groups confirming that non-TIR sequences might be of more ancient origin than TIR sequences.

KW - Apricot

KW - non-TIR-NBS-LRR

KW - Prunus armeniaca L.

KW - resistance gene analogs

KW - RGA

UR - http://www.scopus.com/inward/record.url?scp=79956152042&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956152042&partnerID=8YFLogxK

U2 - 10.1556/ABiol.62.2011.2.7

DO - 10.1556/ABiol.62.2011.2.7

M3 - Article

VL - 62

SP - 171

EP - 181

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 2

ER -