Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers

A. Mihalyi, O. Gevaert, C. M. Kyama, P. Simsa, N. Pochet, F. De Smet, B. De Moor, C. Meuleman, J. Billen, N. Blanckaert, A. Vodolazkaia, V. Fülöp, T. M. D'Hooghe

Research output: Contribution to journalArticle

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Abstract

Background: Lack of a non-invasive diagnostic test contributes to the long delay between onset of symptoms and diagnosis of endometriosis. The aim of this study was to evaluate the combined performance of six potential plasma biomarkers in the diagnosis of endometriosis. Methods: This case-control study was conducted in 294 infertile women, consisting of 93 women with a normal pelvis and 201 women with endometriosis. We measured plasma concentrations of interleukin (IL)-6, IL-8, tumour necrosis factor-alpha, high-sensitivity C-reactive protein (hsCRP), and cancer antigens CA-125 and CA-19-9. Analyses were done using the Kruskal-Wallis test, Mann-Whitney test, receiver operator characteristic, stepwise logistic regression and least squares support vector machines (LSSVM). Results: Plasma levels of IL-6, IL-8 and CA-125 were increased in all women with endometriosis and in those with minimal-mild endometriosis, compared with controls. In women with moderate-severe endometriosis, plasma levels of IL-6, IL-8 and CA-125, but also of hsCRP, were significantly higher than in controls. Using stepwise logistic regression, moderate-severe endometriosis was diagnosed with a sensitivity of 100 (specificity 84) and minimal-mild endometriosis was detected with a sensitivity of 87 (specificity 71) during the secretory phase. Using LSSVM analysis, minimal-mild endometriosis was diagnosed with a sensitivity of 94 (specificity 61) during the secretory phase and with a sensitivity of 92 (specificity 63) during the menstrual phase. Conclusion: SAdvanced statistical analysis of a panel of six selected plasma biomarkers on samples obtained during the secretory phase or during menstruation allows the diagnosis of both minimal-mild and moderate-severe endometriosis with high sensitivity and clinically acceptable specificity.

Original languageEnglish
Pages (from-to)654-664
Number of pages11
JournalHuman Reproduction
Volume25
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Endometriosis
Biomarkers
Interleukin-8
Sensitivity and Specificity
Interleukin-6
Least-Squares Analysis
C-Reactive Protein
CA-125 Antigen
Logistic Models
Menstruation
Pelvis
Routine Diagnostic Tests
Case-Control Studies
Tumor Necrosis Factor-alpha

Keywords

  • Endometriosis
  • Menstrual phase
  • Non-invasive diagnosis
  • Plasma biomarkers
  • Secretory

ASJC Scopus subject areas

  • Rehabilitation
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Mihalyi, A., Gevaert, O., Kyama, C. M., Simsa, P., Pochet, N., De Smet, F., ... D'Hooghe, T. M. (2010). Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers. Human Reproduction, 25(3), 654-664. https://doi.org/10.1093/humrep/dep425

Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers. / Mihalyi, A.; Gevaert, O.; Kyama, C. M.; Simsa, P.; Pochet, N.; De Smet, F.; De Moor, B.; Meuleman, C.; Billen, J.; Blanckaert, N.; Vodolazkaia, A.; Fülöp, V.; D'Hooghe, T. M.

In: Human Reproduction, Vol. 25, No. 3, 03.2010, p. 654-664.

Research output: Contribution to journalArticle

Mihalyi, A, Gevaert, O, Kyama, CM, Simsa, P, Pochet, N, De Smet, F, De Moor, B, Meuleman, C, Billen, J, Blanckaert, N, Vodolazkaia, A, Fülöp, V & D'Hooghe, TM 2010, 'Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers', Human Reproduction, vol. 25, no. 3, pp. 654-664. https://doi.org/10.1093/humrep/dep425
Mihalyi, A. ; Gevaert, O. ; Kyama, C. M. ; Simsa, P. ; Pochet, N. ; De Smet, F. ; De Moor, B. ; Meuleman, C. ; Billen, J. ; Blanckaert, N. ; Vodolazkaia, A. ; Fülöp, V. ; D'Hooghe, T. M. / Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers. In: Human Reproduction. 2010 ; Vol. 25, No. 3. pp. 654-664.
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AU - Kyama, C. M.

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AU - Pochet, N.

AU - De Smet, F.

AU - De Moor, B.

AU - Meuleman, C.

AU - Billen, J.

AU - Blanckaert, N.

AU - Vodolazkaia, A.

AU - Fülöp, V.

AU - D'Hooghe, T. M.

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