Non-fibrillar β-amyloid abates spike-timing-dependent synaptic potentiation at excitatory synapses in layer 2/3 of the neocortex by targeting postsynaptic AMPA receptors

Isaac Shemer, Carl Holmgren, Rogier Min, Livia Fülöp, Misha Zilberter, Kyle M. Sousa, Tamás Farkas, Wolfgang Härtig, Botond Penke, Nail Burnashev, Heikki Tanila, Yuri Zilberter, Tibor Harkany

Research output: Contribution to journalArticle

62 Citations (Scopus)


Cognitive decline in Alzheimer's disease (AD) stems from the progressive dysfunction of synaptic connections within cortical neuronal microcircuits. Recently, soluble amyloid β protein oligomers (Aβols) have been identified as critical triggers for early synaptic disorganization. However, it remains unknown whether a deficit of Hebbian-related synaptic plasticity occurs during the early phase of AD. Therefore, we studied whether age-dependent Aβ accumulation affects the induction of spike-timing-dependent synaptic potentiation at excitatory synapses on neocortical layer 2/3 (L2/3) pyramidal cells in the APPswe/PS1dE9 transgenic mouse model of AD. Synaptic potentiation at excitatory synapses onto L2/3 pyramidal cells was significantly reduced at the onset of Aβ pathology and was virtually absent in mice with advanced Aβ burden. A decreased α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)/N-methyl-d-aspartate (NMDA) receptor-mediated current ratio implicated postsynaptic mechanisms underlying Aβ synaptotoxicity. The integral role of Aβols in these processes was verified by showing that pretreatment of cortical slices with Aβ(25-35)ols disrupted spike-timing-dependent synaptic potentiation at unitary connections between L2/3 pyramidal cells, and reduced the amplitude of miniature excitatory postsynaptic currents therein. A robust decrement of AMPA, but not NMDA, receptor-mediated currents in nucleated patches from L2/3 pyramidal cells confirmed that Aβols perturb basal glutamatergic synaptic transmission by affecting postsynaptic AMPA receptors. Inhibition of AMPA receptor desensitization by cyclothiazide significantly increased the amplitude of excitatory postsynaptic potentials evoked by afferent stimulation, and rescued synaptic plasticity even in mice with pronounced Aβ pathology. We propose that soluble Aβols trigger the diminution of synaptic plasticity in neocortical pyramidal cell networks during early stages of AD pathogenesis by preferentially targeting postsynaptic AMPA receptors.

Original languageEnglish
Pages (from-to)2035-2047
Number of pages13
JournalEuropean Journal of Neuroscience
Issue number8
Publication statusPublished - Apr 1 2006


  • Alzheimer's disease
  • Excitatory synapse
  • Neocortex
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

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