Non-enzymatic antioxidant accumulations in BR-deficient and BR-insensitive barley mutants under control and drought conditions

Damian Gruszka, Anna Janeczko, Michal Dziurka, Ewa Pociecha, J. Fodor

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Drought is one of the most adverse stresses that affect plant growth and yield. Disturbances in metabolic activity resulting from drought cause overproduction of reactive oxygen species. It is postulated that brassinosteroids (BRs) regulate plant tolerance to the stress conditions, but the underlying mechanisms remain largely unknown. An involvement of endogenous BRs in regulation of the antioxidant homeostasis is not fully clarified either. Therefore, the aim of this study was to elucidate the role of endogenous BRs in regulation of non-enzymatic antioxidants in barley (Hordeum vulgare) under control and drought conditions. The plant material included the ‘Bowman’ cultivar and a group of semi-dwarf near-isogenic lines (NILs), representing mutants deficient in BR biosynthesis or signaling. In general, accumulations of 11 compounds representing various types of non-enzymatic antioxidants were analyzed under both conditions. The analyses of accumulations of reduced and oxidized forms of ascorbate indicated that the BR mutants contain significantly higher contents of dehydroascorbic acid under drought conditions when compared with the ‘Bowman’ cultivar. The analysis of glutathione accumulation indicated that under the control conditions the BR-insensitive NILs contained significantly lower concentrations of this antioxidant when compared with the rest of genotypes. Therefore, we postulate that BR sensitivity is required for normal accumulation of glutathione. A complete accumulation profile of various tocopherols indicated that functional BR biosynthesis and signaling are required for their normal accumulation under both conditions. Results of this study provided an insight into the role of endogenous BRs in regulation of the non-enzymatic antioxidant homeostasis.

Original languageEnglish
Pages (from-to)155-169
Number of pages15
JournalPhysiologia Plantarum
Volume163
Issue number2
DOIs
Publication statusPublished - Jun 1 2018

Fingerprint

Brassinosteroids
brassinosteroids
Droughts
Hordeum
Antioxidants
barley
drought
antioxidants
mutants
isogenic lines
Glutathione
glutathione
homeostasis
Homeostasis
Dehydroascorbic Acid
biosynthesis
dehydroascorbic acid
Tocopherols
cultivars
tocopherols

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Non-enzymatic antioxidant accumulations in BR-deficient and BR-insensitive barley mutants under control and drought conditions. / Gruszka, Damian; Janeczko, Anna; Dziurka, Michal; Pociecha, Ewa; Fodor, J.

In: Physiologia Plantarum, Vol. 163, No. 2, 01.06.2018, p. 155-169.

Research output: Contribution to journalArticle

Gruszka, Damian ; Janeczko, Anna ; Dziurka, Michal ; Pociecha, Ewa ; Fodor, J. / Non-enzymatic antioxidant accumulations in BR-deficient and BR-insensitive barley mutants under control and drought conditions. In: Physiologia Plantarum. 2018 ; Vol. 163, No. 2. pp. 155-169.
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