Non-destructive method of frog (Rana esculenta L.) skeleton elemental analysis used during environmental assessment

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Amphibians, particularly frogs, are increasingly used as bioindicators of contaminant accumulation in pollution studies. Their use for field scientific purposes is limited because most frog species are endangered and protected in most countries; killing of specimens are not allowed. The aim of our study was to work out and assess a method by which the elemental contents of frog bones could be estimated effectively based on the toe bones. For this purpose, Rana esculenta L. individuals were collected from an urban pond in Debrecen city (Hungary). The following large bones were also analysed: Skull, spinal, femur, tibia-fibula, tarsal bones, metatarsus, humerus and digits from front and hind limbs. In the bones, P, Ca, Mg, Mn, Na, S and Zn elements were measured. The elemental contents of large bones were significantly correlated with bones weight in the case of each element. The elemental contents of phalanges were also estimated based on the large bones. The measured and the estimated elemental contents of phalanges were not different significantly based on the tibia-fibula, metatarsalis bones, front and hind limb digits. Elemental analysis based on phalanges adds a further way of use of phalanges. Frogs using their phalanges could be useful indicators in the assessment of environmental contamination.

Original languageEnglish
Pages (from-to)467-471
Number of pages5
JournalWater, Air, and Soil Pollution
Volume209
Issue number1-4
DOIs
Publication statusPublished - Jun 2010

Fingerprint

environmental assessment
frog
skeleton
bone
Bone
Chemical analysis
limb
analysis
method
Environmental assessments
Ponds
Biomarkers
skull
bioindicator
endangered species
amphibian
Pollution
Contamination
pond
Impurities

Keywords

  • Bone analysis
  • Environmental indicators
  • Heavy metal
  • Rana esculenta L.
  • Toe bones
  • Toe clipping

ASJC Scopus subject areas

  • Pollution
  • Environmental Chemistry
  • Environmental Engineering
  • Ecological Modelling
  • Water Science and Technology

Cite this

@article{85c7077c68b44af782ee2cdbf32ace1c,
title = "Non-destructive method of frog (Rana esculenta L.) skeleton elemental analysis used during environmental assessment",
abstract = "Amphibians, particularly frogs, are increasingly used as bioindicators of contaminant accumulation in pollution studies. Their use for field scientific purposes is limited because most frog species are endangered and protected in most countries; killing of specimens are not allowed. The aim of our study was to work out and assess a method by which the elemental contents of frog bones could be estimated effectively based on the toe bones. For this purpose, Rana esculenta L. individuals were collected from an urban pond in Debrecen city (Hungary). The following large bones were also analysed: Skull, spinal, femur, tibia-fibula, tarsal bones, metatarsus, humerus and digits from front and hind limbs. In the bones, P, Ca, Mg, Mn, Na, S and Zn elements were measured. The elemental contents of large bones were significantly correlated with bones weight in the case of each element. The elemental contents of phalanges were also estimated based on the large bones. The measured and the estimated elemental contents of phalanges were not different significantly based on the tibia-fibula, metatarsalis bones, front and hind limb digits. Elemental analysis based on phalanges adds a further way of use of phalanges. Frogs using their phalanges could be useful indicators in the assessment of environmental contamination.",
keywords = "Bone analysis, Environmental indicators, Heavy metal, Rana esculenta L., Toe bones, Toe clipping",
author = "Edina Simon and M. Braun and B. T{\'o}thm{\'e}r{\'e}sz",
year = "2010",
month = "6",
doi = "10.1007/s11270-009-0214-6",
language = "English",
volume = "209",
pages = "467--471",
journal = "Water, Air, and Soil Pollution",
issn = "0049-6979",
publisher = "Springer Netherlands",
number = "1-4",

}

TY - JOUR

T1 - Non-destructive method of frog (Rana esculenta L.) skeleton elemental analysis used during environmental assessment

AU - Simon, Edina

AU - Braun, M.

AU - Tóthmérész, B.

PY - 2010/6

Y1 - 2010/6

N2 - Amphibians, particularly frogs, are increasingly used as bioindicators of contaminant accumulation in pollution studies. Their use for field scientific purposes is limited because most frog species are endangered and protected in most countries; killing of specimens are not allowed. The aim of our study was to work out and assess a method by which the elemental contents of frog bones could be estimated effectively based on the toe bones. For this purpose, Rana esculenta L. individuals were collected from an urban pond in Debrecen city (Hungary). The following large bones were also analysed: Skull, spinal, femur, tibia-fibula, tarsal bones, metatarsus, humerus and digits from front and hind limbs. In the bones, P, Ca, Mg, Mn, Na, S and Zn elements were measured. The elemental contents of large bones were significantly correlated with bones weight in the case of each element. The elemental contents of phalanges were also estimated based on the large bones. The measured and the estimated elemental contents of phalanges were not different significantly based on the tibia-fibula, metatarsalis bones, front and hind limb digits. Elemental analysis based on phalanges adds a further way of use of phalanges. Frogs using their phalanges could be useful indicators in the assessment of environmental contamination.

AB - Amphibians, particularly frogs, are increasingly used as bioindicators of contaminant accumulation in pollution studies. Their use for field scientific purposes is limited because most frog species are endangered and protected in most countries; killing of specimens are not allowed. The aim of our study was to work out and assess a method by which the elemental contents of frog bones could be estimated effectively based on the toe bones. For this purpose, Rana esculenta L. individuals were collected from an urban pond in Debrecen city (Hungary). The following large bones were also analysed: Skull, spinal, femur, tibia-fibula, tarsal bones, metatarsus, humerus and digits from front and hind limbs. In the bones, P, Ca, Mg, Mn, Na, S and Zn elements were measured. The elemental contents of large bones were significantly correlated with bones weight in the case of each element. The elemental contents of phalanges were also estimated based on the large bones. The measured and the estimated elemental contents of phalanges were not different significantly based on the tibia-fibula, metatarsalis bones, front and hind limb digits. Elemental analysis based on phalanges adds a further way of use of phalanges. Frogs using their phalanges could be useful indicators in the assessment of environmental contamination.

KW - Bone analysis

KW - Environmental indicators

KW - Heavy metal

KW - Rana esculenta L.

KW - Toe bones

KW - Toe clipping

UR - http://www.scopus.com/inward/record.url?scp=77952236238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952236238&partnerID=8YFLogxK

U2 - 10.1007/s11270-009-0214-6

DO - 10.1007/s11270-009-0214-6

M3 - Article

AN - SCOPUS:77952236238

VL - 209

SP - 467

EP - 471

JO - Water, Air, and Soil Pollution

JF - Water, Air, and Soil Pollution

SN - 0049-6979

IS - 1-4

ER -