Non-antibody components in porcine anti-DNP antibody preparations obtained by affinity chromatography

František Franěk, Mohamed A. Saber, Jiři Doskočil, Josef Novotný, G. Füst

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Porcine anti-DNP antibody preparations obtained by immunoadsorption on Sepharose-bound dinitrophenylated serum albumin and elution by a dinitrophenol solution were found to contain high-mol. wt protein components in addition to specific anti-DNP antibodies of the immunoglobulin G class. The high-mol. wt components were resolved by gel chromatography into three fractions. Antigenic analysis and assays of antibody activity with the aid of chemically modified bacteriophage revealed the presence of immunoglobulin M having neither anti-DNP nor anti-immunoglobulin activity, and the presence of several non-immunoglobulin components. These components, mutually antigenically related, were present in all fractions obtained by gel chromatography. The non-immunoglobulin components were antigenically related to a protein having β-globulin electrophoretic mobility occurring in pig serum. The amino acid composition of the main non-immunoglobulin fraction (mol. wt 520,000) displayed a certain relatedness to the composition of the C3 and C4 components of complement. The appearance of the non-immunoglobulin proteins in anti-DNP antibody preparations could not be prevented by addition of EDTA to the anti-DNP serum. The non-immunoglobulin proteins were virtually absent in the anti-DNP antibody preparation obtained from a serum that had been treated with an unrelated antigen-antibody precipitate.

Original languageEnglish
Pages (from-to)389-394
Number of pages6
JournalMolecular Immunology
Volume16
Issue number6
DOIs
Publication statusPublished - 1979

Fingerprint

Affinity Chromatography
Anti-Idiotypic Antibodies
Swine
Gel Chromatography
Proteins
Serum
Complement C4
Dinitrophenols
Antibodies
Immunoglobulin Isotypes
Globulins
Serum Albumin
Edetic Acid
Bacteriophages
Sepharose
Immunoglobulin M
Immunoglobulins
Immunoglobulin G
Antigens
Amino Acids

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Non-antibody components in porcine anti-DNP antibody preparations obtained by affinity chromatography. / Franěk, František; Saber, Mohamed A.; Doskočil, Jiři; Novotný, Josef; Füst, G.

In: Molecular Immunology, Vol. 16, No. 6, 1979, p. 389-394.

Research output: Contribution to journalArticle

Franěk, František ; Saber, Mohamed A. ; Doskočil, Jiři ; Novotný, Josef ; Füst, G. / Non-antibody components in porcine anti-DNP antibody preparations obtained by affinity chromatography. In: Molecular Immunology. 1979 ; Vol. 16, No. 6. pp. 389-394.
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AU - Füst, G.

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