Non-adrenergic non-cholinergic neuron stimulation in the cat lower esophageal sphincter

Nadejda Kortezova, Zlatka Mizhorkova, Elissaveta Milusheva, G. Varga, E. Vízi, Maria Papasova

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Both electrical field stimulation and nicotine produced non-adrenergic non-cholinergic (NANC) relaxation of the circular muscle strips from the cat lower esophageal sphincter in the presence of 5 μM guanethidine and 5 μM scopolamine. Low-frequency stimulation (2 Hz, 0.2 ms duration, supramaximal current intensity, 20-s train) provoked a transient relaxation, while at high-frequency stimulation (20 Hz) a slow restoration to the resting tone was observed. Blockade of nitric oxide (NO) synthesis by 1 mM N(ω)-nitro-L-arginine decreased by 20% the amplitude of the 20 Hz-induced relaxation and changed the pattern of relaxation, making it similar to the sustained relaxation evoked by exogenously applied vasoactive intestinal peptide (VIP). After chymotrypsin (4 U/ml), the pattern of the high-frequency-induced relaxation resembled that of the low-frequency-induced relaxation. Similarly, chymotrypsin changed the shape of nicotine-provoked relaxation, increasing the speed of restoration to the resting tone. We suggest that the fast relaxation elicited in cat lower esophageal sphincter by electrical field stimulation or nicotine is initiated by NO. The slow restoration to the resting tone in the case of high-frequency- or nicotine-induced relaxation seems to be due to the release of VIP or VIP-like peptides. The possibility of participation of another transmitter(s) involved in NANC relaxation should not be excluded.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalEuropean Journal of Pharmacology
Volume304
Issue number1-3
DOIs
Publication statusPublished - May 23 1996

Fingerprint

Lower Esophageal Sphincter
Nicotine
Vasoactive Intestinal Peptide
Cats
Neurons
Chymotrypsin
Electric Stimulation
Nitric Oxide
Guanethidine
Muscle Relaxation
Scopolamine Hydrobromide
Arginine
Peptides

Keywords

  • electrical field stimulation
  • lower esophageal sphincter
  • NANC (non-adrenergic non-cholinergic) neurotransmission
  • nicotine
  • nitric oxide (NO)
  • VIP (vasoactive intestinal peptide)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Non-adrenergic non-cholinergic neuron stimulation in the cat lower esophageal sphincter. / Kortezova, Nadejda; Mizhorkova, Zlatka; Milusheva, Elissaveta; Varga, G.; Vízi, E.; Papasova, Maria.

In: European Journal of Pharmacology, Vol. 304, No. 1-3, 23.05.1996, p. 109-115.

Research output: Contribution to journalArticle

Kortezova, Nadejda ; Mizhorkova, Zlatka ; Milusheva, Elissaveta ; Varga, G. ; Vízi, E. ; Papasova, Maria. / Non-adrenergic non-cholinergic neuron stimulation in the cat lower esophageal sphincter. In: European Journal of Pharmacology. 1996 ; Vol. 304, No. 1-3. pp. 109-115.
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