Nodule-specific cysteine-rich peptides negatively regulate nitrogen-fixing symbiosis in a strain-specific manner in medicago truncatul

Qi Wang, Jinge Liu, Hua Li, Shengming Yang, Peter Körmöczi, A. Kereszt, Hongyan Zhu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Medicago truncatula shows a high level of specificity when interacting with its symbiotic partner Sinorhizobium meliloti. This specificity is mainly manifested at the nitrogen-fixing stage of nodule development, such that a particular bacterial strain forms nitrogen-fixing nodules (NodiFix) on one plant genotype but ineffective nodules (NodIFix ) on another. Recent studies have just begun to reveal the underlying molecular mechanisms that control this specificity. The S. meliloti strain A145 induces the formation of Fix nodules on the accession DZA315.16 but Fix nodules on Jemalong A17. A previous study reported that the formation of Fix nodules on Jemalong A17 by S. meliloti A145 was conditioned by a single recessive allele named Mtsym6. Here we demonstrate that the specificity associated with S. meliloti A145 is controlled by multiple genes in M. truncatula, including NFS1 and NFS2 that encode nodule-specific cysteinerich (NCR) peptides. The two NCR peptides acted dominantly to block rather than promote nitrogen fixation by S. meliloti A145. These two NCR peptides are the same ones that negatively regulate nitrogen-fixing symbiosis associated with S. meliloti Rm41.

Original languageEnglish
Pages (from-to)240-248
Number of pages9
JournalMolecular Plant-Microbe Interactions
Volume31
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

Sinorhizobium meliloti
N-ethylcarbaminomethyl-L-isoleucine
Medicago
Ensifer meliloti
Symbiosis
symbiosis
Cysteine
cysteine
Nitrogen
peptides
Peptides
nitrogen
Medicago truncatula
A 17
Nitrogen Fixation
nitrogen fixation
Alleles
Genotype
alleles
genotype

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Nodule-specific cysteine-rich peptides negatively regulate nitrogen-fixing symbiosis in a strain-specific manner in medicago truncatul. / Wang, Qi; Liu, Jinge; Li, Hua; Yang, Shengming; Körmöczi, Peter; Kereszt, A.; Zhu, Hongyan.

In: Molecular Plant-Microbe Interactions, Vol. 31, No. 2, 01.02.2018, p. 240-248.

Research output: Contribution to journalArticle

Wang, Qi ; Liu, Jinge ; Li, Hua ; Yang, Shengming ; Körmöczi, Peter ; Kereszt, A. ; Zhu, Hongyan. / Nodule-specific cysteine-rich peptides negatively regulate nitrogen-fixing symbiosis in a strain-specific manner in medicago truncatul. In: Molecular Plant-Microbe Interactions. 2018 ; Vol. 31, No. 2. pp. 240-248.
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