Nodule-expressed Cyp15a cysteine protease genes map to syntenic genome regions in Pisum and Medicago spp.

Jason L. Vincent, Maggie R. Knox, T. H. Noel Ellis, Péter Kaló, György B. Kiss, Nicholas J. Brewin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

PsCyp15a is a gene that encodes a vacuolar cysteine protease expressed in wilt-induced shoots of Pisum sativum (pea) and in root nodules. To further the understanding of nodular PsCyp15a expression, a region 5' to the coding sequence of the gene was cloned. Varying lengths of 5' untranslated sequence were fused with the uidA coding region and introduced from Agrobacterium rhizogenes into 'hairy roots' of Vicia hirsuta. In this transgenic root nodulation assay, a promoter sequence of 900 bp was sufficient to give an expression pattern indistinguishable from that obtained in pea nodules by in situ hybridization. An orthologue of PsCyp15a was cloned from nodule mRNA of Medicago sativa and a corresponding gene identified in M. truncatula was also shown to express strongly in nodules. With molecular mapping techniques, it was demonstrated that these genes map to a syntenic genome location in pea and Medicago spp., but the map positions of the Cyp15a genes cannot be correlated with existing nodulation mutants.

Original languageEnglish
Pages (from-to)715-723
Number of pages9
JournalMolecular Plant-Microbe Interactions
Volume13
Issue number7
DOIs
Publication statusPublished - Jul 2000

Keywords

  • Transformation

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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