Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators

Péter Kaló, Cynthia Gleason, Anne Edwards, John Marsh, Raka M. Mitra, Sibylle Hirsch, Júlia Jakab, Sarah Sims, Sharon R. Long, Jane Rogers, György B. Kiss, J. Allan Downie, Giles E.D. Oldroyd

Research output: Contribution to journalArticle

366 Citations (Scopus)

Abstract

Rhizobial bacteria enter a symbiotic interaction with legumes, activating diverse responses in roots through the lipochito oligosaccharide signaling molecule Nod factor. Here, we show that NSP2 from Medicago truncatula encodes a GRAS protein essential for Nod-factor signaling. NSP2 functions downstream of Nod-factor-induced calcium spiking and a calcium/calmodulin-dependent protein kinase. We show that NSP2-GFP expressed from a constitutive promoter is localized to the endoplasmic reticulum/nuclear envelope and relocalizes to the nucleus after Nod-factor elicitation. This work provides evidence that a GRAS protein transduces calcium signals in plants and provides a possible regulator of Nod-factor-inducible gene expression.

Original languageEnglish
Pages (from-to)1786-1789
Number of pages4
JournalScience
Volume308
Issue number5729
DOIs
Publication statusPublished - Jun 17 2005

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators'. Together they form a unique fingerprint.

  • Cite this

    Kaló, P., Gleason, C., Edwards, A., Marsh, J., Mitra, R. M., Hirsch, S., Jakab, J., Sims, S., Long, S. R., Rogers, J., Kiss, G. B., Downie, J. A., & Oldroyd, G. E. D. (2005). Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators. Science, 308(5729), 1786-1789. https://doi.org/10.1126/science.1110951