Nociception, neurogenic inflammation and thermoregulation in TRPV1 knockdown transgenic mice

Dániel Márton Tóth, Éva Szoke, Kata Bölcskei, Krisztián Kvell, Balázs Bender, Zsuzsanna Bosze, János Szolcsányi, Zoltán Sándor

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Transgenic mice with a small hairpin RNA construct interfering with the expression of transient receptor potential vanilloid 1 (TRPV1) were created by lentiviral transgenesis. TRPV1 expression level in transgenic mice was reduced to 8% while the expression of ankyrin repeat domain 1 (TRPA1) was unchanged. Ear oedema induced by topical application of TRPV1 agonist capsaicin was completely absent in TRPV1 knockdown mice. Thermoregulatory behaviour in relation to environmental thermopreference (30 vs. 35°C) was slightly impaired in male knockdown mice, but the reduction of TRPV1 function was not associated with enhanced hyperthermia. TRPV1 agonist resiniferatoxin induced hypothermia and tail vasodilatation was markedly inhibited in knockdown mice. In conclusion, shRNA-mediated knock down of the TRPV1 receptor in mice induced robust inhibition of the responses to TRPV1 agonists without altering the expression, gating function or neurogenic oedema provoked by TRPA1 activation. Thermoregulatory behaviour in response to heat was inhibited, but enhanced hyperthermia was not observed.

Original languageEnglish
Pages (from-to)2589-2601
Number of pages13
JournalCellular and Molecular Life Sciences
Volume68
Issue number15
DOIs
Publication statusPublished - Aug 1 2011

Keywords

  • Knockdown
  • Lentiviral transgenesis
  • Neurogenic inflammation
  • RNA interference
  • TRPA1
  • TRPV1
  • Thermoregulation

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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