Nocardioides hungaricus sp. nov., isolated from a drinking water supply system

E. Tóth, Zsuzsa Kéki, Judit Makk, Zalán G. Homonnay, K. Márialigeti, Peter Schumann

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Three Gram-positive, rod-shaped bacterial strains were isolated from the drinking water supply system of the Hungarian capital, Budapest. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the isolates represented a distinct cluster within the clade of the genus Nocardioides and were most closely related to Nocardioides pyridinolyticus OS4T, Nocardioides aquiterrae GW-9T, Nocardioides sediminis MSL-01T and N. hankookensis DS-30T. The peptidoglycan based on LL-2,6-diaminopimelic acid, the major menaquinone MK-8(H4), the cellular fatty acid profile with iso-C16 : 0 and anteiso-C17 : 0 as predominating components and the DNA G+C content of 71.4 mol% (strain 1RaM5-12T) were consistent with the affiliation of the isolates to the genus Nocardioides. Because of differences in physiological characteristics, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of protein extracts, PvuII RiboPrinter patterns and 96.1% 16S rRNA gene sequence similarity between strain 1RaM5-12T and its closest phylogenetic neighbour, N. pyridinolyticus OS4T, a novel species, Nocardioides hungaricus sp. nov., is proposed. The type strain is 1RaM5-12T (=DSM 21673T =NCAIM 02330T).

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number3
DOIs
Publication statusPublished - Mar 2011

Fingerprint

Nocardioides
Water Supply
rRNA Genes
Nocardioides pyridinolyticus
Drinking Water
water supply
drinking water
Gram-Positive Rods
Diaminopimelic Acid
phylogenetics
Peptidoglycan
gene
new species
Base Composition
desorption
Lasers
ionization
Fatty Acids
fatty acid
laser

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Nocardioides hungaricus sp. nov., isolated from a drinking water supply system. / Tóth, E.; Kéki, Zsuzsa; Makk, Judit; Homonnay, Zalán G.; Márialigeti, K.; Schumann, Peter.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 61, No. 3, 03.2011, p. 549-553.

Research output: Contribution to journalArticle

Tóth, E. ; Kéki, Zsuzsa ; Makk, Judit ; Homonnay, Zalán G. ; Márialigeti, K. ; Schumann, Peter. / Nocardioides hungaricus sp. nov., isolated from a drinking water supply system. In: International Journal of Systematic and Evolutionary Microbiology. 2011 ; Vol. 61, No. 3. pp. 549-553.
@article{8426cd67472c4f3280757f7c3d568a42,
title = "Nocardioides hungaricus sp. nov., isolated from a drinking water supply system",
abstract = "Three Gram-positive, rod-shaped bacterial strains were isolated from the drinking water supply system of the Hungarian capital, Budapest. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the isolates represented a distinct cluster within the clade of the genus Nocardioides and were most closely related to Nocardioides pyridinolyticus OS4T, Nocardioides aquiterrae GW-9T, Nocardioides sediminis MSL-01T and N. hankookensis DS-30T. The peptidoglycan based on LL-2,6-diaminopimelic acid, the major menaquinone MK-8(H4), the cellular fatty acid profile with iso-C16 : 0 and anteiso-C17 : 0 as predominating components and the DNA G+C content of 71.4 mol{\%} (strain 1RaM5-12T) were consistent with the affiliation of the isolates to the genus Nocardioides. Because of differences in physiological characteristics, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of protein extracts, PvuII RiboPrinter patterns and 96.1{\%} 16S rRNA gene sequence similarity between strain 1RaM5-12T and its closest phylogenetic neighbour, N. pyridinolyticus OS4T, a novel species, Nocardioides hungaricus sp. nov., is proposed. The type strain is 1RaM5-12T (=DSM 21673T =NCAIM 02330T).",
author = "E. T{\'o}th and Zsuzsa K{\'e}ki and Judit Makk and Homonnay, {Zal{\'a}n G.} and K. M{\'a}rialigeti and Peter Schumann",
year = "2011",
month = "3",
doi = "10.1099/ijs.0.022939-0",
language = "English",
volume = "61",
pages = "549--553",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "3",

}

TY - JOUR

T1 - Nocardioides hungaricus sp. nov., isolated from a drinking water supply system

AU - Tóth, E.

AU - Kéki, Zsuzsa

AU - Makk, Judit

AU - Homonnay, Zalán G.

AU - Márialigeti, K.

AU - Schumann, Peter

PY - 2011/3

Y1 - 2011/3

N2 - Three Gram-positive, rod-shaped bacterial strains were isolated from the drinking water supply system of the Hungarian capital, Budapest. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the isolates represented a distinct cluster within the clade of the genus Nocardioides and were most closely related to Nocardioides pyridinolyticus OS4T, Nocardioides aquiterrae GW-9T, Nocardioides sediminis MSL-01T and N. hankookensis DS-30T. The peptidoglycan based on LL-2,6-diaminopimelic acid, the major menaquinone MK-8(H4), the cellular fatty acid profile with iso-C16 : 0 and anteiso-C17 : 0 as predominating components and the DNA G+C content of 71.4 mol% (strain 1RaM5-12T) were consistent with the affiliation of the isolates to the genus Nocardioides. Because of differences in physiological characteristics, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of protein extracts, PvuII RiboPrinter patterns and 96.1% 16S rRNA gene sequence similarity between strain 1RaM5-12T and its closest phylogenetic neighbour, N. pyridinolyticus OS4T, a novel species, Nocardioides hungaricus sp. nov., is proposed. The type strain is 1RaM5-12T (=DSM 21673T =NCAIM 02330T).

AB - Three Gram-positive, rod-shaped bacterial strains were isolated from the drinking water supply system of the Hungarian capital, Budapest. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the isolates represented a distinct cluster within the clade of the genus Nocardioides and were most closely related to Nocardioides pyridinolyticus OS4T, Nocardioides aquiterrae GW-9T, Nocardioides sediminis MSL-01T and N. hankookensis DS-30T. The peptidoglycan based on LL-2,6-diaminopimelic acid, the major menaquinone MK-8(H4), the cellular fatty acid profile with iso-C16 : 0 and anteiso-C17 : 0 as predominating components and the DNA G+C content of 71.4 mol% (strain 1RaM5-12T) were consistent with the affiliation of the isolates to the genus Nocardioides. Because of differences in physiological characteristics, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of protein extracts, PvuII RiboPrinter patterns and 96.1% 16S rRNA gene sequence similarity between strain 1RaM5-12T and its closest phylogenetic neighbour, N. pyridinolyticus OS4T, a novel species, Nocardioides hungaricus sp. nov., is proposed. The type strain is 1RaM5-12T (=DSM 21673T =NCAIM 02330T).

UR - http://www.scopus.com/inward/record.url?scp=79952392170&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952392170&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.022939-0

DO - 10.1099/ijs.0.022939-0

M3 - Article

C2 - 20382790

AN - SCOPUS:79952392170

VL - 61

SP - 549

EP - 553

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 3

ER -