No mutation but high mRNA expression of Coxsackie-Adenovirus Receptor was observed in both dilated and ischemic cardiomyopathy

Eniko Tatrai, Katalin Bedi, Ilona Kovalszky, Istvan Hartyanszky, Andras Laszik, Gyorgy Acsady, Peter Sotonyi, Marta Hubay

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The most common causes of acute myocarditis and the possible consequence of dilated cardiomyopathy are virus infections. The receptor of the two most common viruses connected to these myocardial diseases was identified as Coxsackie-Adenovirus Receptor. The purpose of this study was to assess Coxsackie-Adenovirus Receptor mRNA expression in the myocardium and search for mutations in the Coxsackie-Adenovirus Receptor gene to compare dilated, inflammatory and ischemic cardiomyopathy with control group. All the myocardial samples were obtained from 35 explanted hearts during heart transplantation, than DNA and RNA were isolated from the muscle samples. cDNA was generated from RNA using reverse transcription, and real-time PCR was performed with relative quantification by β-actin gene as endogenous control. Using DNA extracted from the myocardial samples, we sequenced all the seven exons of the Coxsackie-Adenovirus Receptor gene. Coxsackie-Adenovirus Receptor mRNA expression was higher in both ischemic and dilated cardiomyopathy groups than in inflammatory cardiomyopathy and healthy control groups. Sequencing of CAR gene showed no sign of mutation. Therefore, the sequences result of CAR exons did not show any mutation or polymorphism, that explains a determinant role of CAR in dilated or ischemic CM. Our results suggest that high mRNA expression of Coxsackie-Adenovirus Receptor may support its role in regeneration of the damaged myocardium rather than having any role in viral mediated heart disease.

Original languageEnglish
Pages (from-to)47-50
Number of pages4
JournalForensic Science International
Volume212
Issue number1-3
DOIs
Publication statusPublished - Oct 10 2011

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Keywords

  • Cardiomyopathy
  • Coxsackie-Adenovirus Receptor
  • Myocarditis
  • Virus

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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