N,N-dimethyltryptamine Prevents Renal Ischemia-Reperfusion Injury in a Rat Model

B. Nemes, Katalin Pető, N. Németh, Anita Mester, Zsuzsanna Magyar, Souleiman Ghanem, Viktória Sógor, Bence Tánczos, Ádám Deák, Márk Kállay, László Bidiga, Ede Frecska

Research output: Contribution to journalArticle

Abstract

Background: Ischemia reperfusion (I/R)injury remains one of the most challenging fields of organ transplantation. It is highly associated with the use of expanded criteria donors that might conclude to delayed graft function or early or late graft failure. Objective: To investigate the metabolic, microcirculatory parameters, and histologic changes under the effect of N,N-dimethyltryptamine (DMT)in a renal I/R model in rats. Method: In 26 anesthetized rats both kidneys were exposed. In the control group (n = 6)no other intervention happened. In 20 other animals, the right renal vessels were ligated, and after 60 minutes the right kidney was removed. The left renal vessels were clamped for 60 minutes then released, followed by 120 minutes of reperfusion. In the I/R group (n = 10), there was no additive treatment, while in I/R + DMT group (n = 10)DMT was administered 15 minutes before ischemia. Blood samples were taken, laser Doppler measurement was performed, and both kidneys were evaluated histologically. Results: Microcirculation (blood flux units [BFU])diminished in all groups, but remarkably so in the I/R + DMT group. This group compensated better after the 30th minute of reperfusion. The control and I/R + DMT groups had similar BFUs after 120 minutes of reperfusion, but in the I/R group BFU was higher. Tubular necrosis developed in the I/R and I/R + DMT groups too; it was moderated under DMT effect, and severe without. Histologic injuries were less in I/R + DMT Group compared to non-treated animals. Conclusion: Histologic changes characteristic to I/R injuries were reversible and microcirculation recovered at the end of 120 minutes reperfusion under the administration of DMT. DMT can be used for renoprotection in kidney transplantation.

Original languageEnglish
Pages (from-to)1268-1275
Number of pages8
JournalTransplantation Proceedings
Volume51
Issue number4
DOIs
Publication statusPublished - May 1 2019

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N,N-Dimethyltryptamine
Reperfusion Injury
Reperfusion
Kidney
Ischemia
Microcirculation
Animal Rights
Delayed Graft Function
Organ Transplantation

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

N,N-dimethyltryptamine Prevents Renal Ischemia-Reperfusion Injury in a Rat Model. / Nemes, B.; Pető, Katalin; Németh, N.; Mester, Anita; Magyar, Zsuzsanna; Ghanem, Souleiman; Sógor, Viktória; Tánczos, Bence; Deák, Ádám; Kállay, Márk; Bidiga, László; Frecska, Ede.

In: Transplantation Proceedings, Vol. 51, No. 4, 01.05.2019, p. 1268-1275.

Research output: Contribution to journalArticle

Nemes, B, Pető, K, Németh, N, Mester, A, Magyar, Z, Ghanem, S, Sógor, V, Tánczos, B, Deák, Á, Kállay, M, Bidiga, L & Frecska, E 2019, 'N,N-dimethyltryptamine Prevents Renal Ischemia-Reperfusion Injury in a Rat Model', Transplantation Proceedings, vol. 51, no. 4, pp. 1268-1275. https://doi.org/10.1016/j.transproceed.2019.04.005
Nemes, B. ; Pető, Katalin ; Németh, N. ; Mester, Anita ; Magyar, Zsuzsanna ; Ghanem, Souleiman ; Sógor, Viktória ; Tánczos, Bence ; Deák, Ádám ; Kállay, Márk ; Bidiga, László ; Frecska, Ede. / N,N-dimethyltryptamine Prevents Renal Ischemia-Reperfusion Injury in a Rat Model. In: Transplantation Proceedings. 2019 ; Vol. 51, No. 4. pp. 1268-1275.
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T1 - N,N-dimethyltryptamine Prevents Renal Ischemia-Reperfusion Injury in a Rat Model

AU - Nemes, B.

AU - Pető, Katalin

AU - Németh, N.

AU - Mester, Anita

AU - Magyar, Zsuzsanna

AU - Ghanem, Souleiman

AU - Sógor, Viktória

AU - Tánczos, Bence

AU - Deák, Ádám

AU - Kállay, Márk

AU - Bidiga, László

AU - Frecska, Ede

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Background: Ischemia reperfusion (I/R)injury remains one of the most challenging fields of organ transplantation. It is highly associated with the use of expanded criteria donors that might conclude to delayed graft function or early or late graft failure. Objective: To investigate the metabolic, microcirculatory parameters, and histologic changes under the effect of N,N-dimethyltryptamine (DMT)in a renal I/R model in rats. Method: In 26 anesthetized rats both kidneys were exposed. In the control group (n = 6)no other intervention happened. In 20 other animals, the right renal vessels were ligated, and after 60 minutes the right kidney was removed. The left renal vessels were clamped for 60 minutes then released, followed by 120 minutes of reperfusion. In the I/R group (n = 10), there was no additive treatment, while in I/R + DMT group (n = 10)DMT was administered 15 minutes before ischemia. Blood samples were taken, laser Doppler measurement was performed, and both kidneys were evaluated histologically. Results: Microcirculation (blood flux units [BFU])diminished in all groups, but remarkably so in the I/R + DMT group. This group compensated better after the 30th minute of reperfusion. The control and I/R + DMT groups had similar BFUs after 120 minutes of reperfusion, but in the I/R group BFU was higher. Tubular necrosis developed in the I/R and I/R + DMT groups too; it was moderated under DMT effect, and severe without. Histologic injuries were less in I/R + DMT Group compared to non-treated animals. Conclusion: Histologic changes characteristic to I/R injuries were reversible and microcirculation recovered at the end of 120 minutes reperfusion under the administration of DMT. DMT can be used for renoprotection in kidney transplantation.

AB - Background: Ischemia reperfusion (I/R)injury remains one of the most challenging fields of organ transplantation. It is highly associated with the use of expanded criteria donors that might conclude to delayed graft function or early or late graft failure. Objective: To investigate the metabolic, microcirculatory parameters, and histologic changes under the effect of N,N-dimethyltryptamine (DMT)in a renal I/R model in rats. Method: In 26 anesthetized rats both kidneys were exposed. In the control group (n = 6)no other intervention happened. In 20 other animals, the right renal vessels were ligated, and after 60 minutes the right kidney was removed. The left renal vessels were clamped for 60 minutes then released, followed by 120 minutes of reperfusion. In the I/R group (n = 10), there was no additive treatment, while in I/R + DMT group (n = 10)DMT was administered 15 minutes before ischemia. Blood samples were taken, laser Doppler measurement was performed, and both kidneys were evaluated histologically. Results: Microcirculation (blood flux units [BFU])diminished in all groups, but remarkably so in the I/R + DMT group. This group compensated better after the 30th minute of reperfusion. The control and I/R + DMT groups had similar BFUs after 120 minutes of reperfusion, but in the I/R group BFU was higher. Tubular necrosis developed in the I/R and I/R + DMT groups too; it was moderated under DMT effect, and severe without. Histologic injuries were less in I/R + DMT Group compared to non-treated animals. Conclusion: Histologic changes characteristic to I/R injuries were reversible and microcirculation recovered at the end of 120 minutes reperfusion under the administration of DMT. DMT can be used for renoprotection in kidney transplantation.

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