Studies on the biomass and alkaloid production of in vitro and in vivo cultures from Lobelia inflata L. proved that these organized cultures are able to synthesize the characteristic alkaloids of the intact. plant. In addition to callus-, organised cultures and hairy root cultures transformed by genetically modified Agrobacterium rhizogenes R-1601 branch were also produced. Isolation by chromatography and spectroscopic characterization and identification (MS, 1H and 13C-NMR) have been our major working tools. Among more than 20 piperidinebase alkaloids (contained in the herb) special attention has been directed to the study of the nicotine agonist lobeline.
|Number of pages||5|
|Journal||Medical Science Monitor|
|Publication status||Published - Jan 1 1998|
- Lobelia inflata
- Piperidine alkaloids
- Tissue culture
ASJC Scopus subject areas