NMDA receptor NR2B subunit over-expression increases cerebellar granule cell migratory activity

Krisztián Tárnok, Katalin Czöndör, Márta Jelitai, A. Czirók, K. Schlett

Research output: Contribution to journalArticle

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Abstract

Glutamate acting on NMDA receptors (NMDARs) is known to influence cerebellar granule cell migration. Subunit composition of NMDARs in granule cells changes characteristically during development: NR2B subunit containing receptors are abundant during migration towards the internal granule cell layer but are gradually replaced by NR2A and/or NR2C subunits once the final position is reached. Cerebellar granule cell migration was investigated using mutant mouse lines either with a deletion of the NR2C gene (NR2C-/- mice) or expressing NR2B instead of the NR2C subunit (NR2C-2B mice). BrdU-labeling revealed that over-expression of NR2B increased granule cell translocation in vivo, while the lack of NR2C subunit did not have any detectable effects on cell migration. Cellular composition of wild-type and mutant dissociated cerebellar granule cell cultures isolated from 10-day-old cerebella were similar, but NR2C-2B cultures had elevated level of NR2B subunits and intracellular Ca 2+ imaging revealed higher sensitivity towards the addition of NR2B-selective antagonist in vitro. Time-lapse videomicroscopic observations revealed that average migratory velocity and the proportion of translocating cell bodies were significantly higher in NR2C-2B than in wild-type cultures. Our results provide evidence that NR2B-containing NMDARs can have specialized roles during granule cell migration and can increase migratory speed.

Original languageEnglish
Pages (from-to)818-829
Number of pages12
JournalJournal of Neurochemistry
Volume104
Issue number3
DOIs
Publication statusPublished - Feb 2008

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N-Methyl-D-Aspartate Receptors
Cell Movement
Cells
Bromodeoxyuridine
Chemical analysis
Cell culture
Labeling
Glutamic Acid
Gene Deletion
Genes
Cerebellum
Imaging techniques
Cell Culture Techniques
NR2B NMDA receptor

Keywords

  • Cerebellum
  • Granule cell
  • Migration
  • NMDA receptor
  • NR2B subunit

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

NMDA receptor NR2B subunit over-expression increases cerebellar granule cell migratory activity. / Tárnok, Krisztián; Czöndör, Katalin; Jelitai, Márta; Czirók, A.; Schlett, K.

In: Journal of Neurochemistry, Vol. 104, No. 3, 02.2008, p. 818-829.

Research output: Contribution to journalArticle

Tárnok, Krisztián ; Czöndör, Katalin ; Jelitai, Márta ; Czirók, A. ; Schlett, K. / NMDA receptor NR2B subunit over-expression increases cerebellar granule cell migratory activity. In: Journal of Neurochemistry. 2008 ; Vol. 104, No. 3. pp. 818-829.
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