Nitrincola alkalilacustris sp. Nov. and nitrincola schmidtii sp. nov., alkaliphilic bacteria isolated from soda pans, and emended description of the genus nitrincola

A. Borsodi, Kristóf Korponai, Peter Schumann, Cathrin Spröer, Tamás Felföldi, K. Márialigeti, Tibor Szili-Kovács, E. Tóth

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Three alkaliphilic and halotolerant bacterial strains, designated ZV-19T, R4-8Tand S4-12, were isolated from the water of soda pans located in the Kiskunság National Park, Hungary. Cells of all three strains were Gram-staining-negative, rodshaped, motile and non-endospore-forming. They were facultatively anaerobic, and oxidase-and catalase-positive. Their major isoprenoid quinone was Q-8, and their predominant fatty acids were C18: 1ω7c, C16: 1ω7c and C16: 0. The DNA G+C content was 54.5 mol% in strain ZV-19 T and 45.8 mol% in strain R4-8T. The 16S rRNA gene based phylogenetic analysis showed that all three strains were members of the genus Nitrincola (family Oceanospirillaceae, class Gammaproteobacteria). Strain ZV-19Tshowed 96.6 and 95.5% sequence similarities and 19±3 and 18±3%DNA-DNA relatedness to Nitrincola lacisaponensis DSM 16316Tand Nitrincola alkalisediminis JCM 19317T, respectively. Strains R4-8Tand S4-12 exhibited 97.9 and 98.6%sequence matches and 34±4 and 13±8%DNA-DNA hybridization values with N. lacisaponensis DSM 16316Tand N. alkalisediminis JCM 19317T, respectively. According to the phenotypic, chemotaxonomic and phylogenetic data, the strains studied represent two novel species, Nitrincola alkalilacustris sp. nov. with the type strain ZV-19T(=DSM 29817T=NCAIM B 02612T) and Nitrincola schmidtii sp. nov. with the type strain R4-8T (=DSM 100788T=NCAIM B.02626T). An emended description of the genus Nitrincola is also presented.

Original languageEnglish
Article number002437
Pages (from-to)5159-5164
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number12
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

aprepitant
Bacteria
DNA
bacterium
new species
bacteria
Oceanospirillaceae
Carbonated Water
phylogenetics
Gammaproteobacteria
Negative Staining
Hungary
isoprenoid
Terpenes
Base Composition
rRNA Genes
relatedness
Catalase
Oxidoreductases
national park

Keywords

  • Alkaliphilic bacterium
  • Nitrincola
  • Soda lake

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Nitrincola alkalilacustris sp. Nov. and nitrincola schmidtii sp. nov., alkaliphilic bacteria isolated from soda pans, and emended description of the genus nitrincola. / Borsodi, A.; Korponai, Kristóf; Schumann, Peter; Spröer, Cathrin; Felföldi, Tamás; Márialigeti, K.; Szili-Kovács, Tibor; Tóth, E.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 12, 002437, 01.12.2017, p. 5159-5164.

Research output: Contribution to journalArticle

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abstract = "Three alkaliphilic and halotolerant bacterial strains, designated ZV-19T, R4-8Tand S4-12, were isolated from the water of soda pans located in the Kiskuns{\'a}g National Park, Hungary. Cells of all three strains were Gram-staining-negative, rodshaped, motile and non-endospore-forming. They were facultatively anaerobic, and oxidase-and catalase-positive. Their major isoprenoid quinone was Q-8, and their predominant fatty acids were C18: 1ω7c, C16: 1ω7c and C16: 0. The DNA G+C content was 54.5 mol{\%} in strain ZV-19 T and 45.8 mol{\%} in strain R4-8T. The 16S rRNA gene based phylogenetic analysis showed that all three strains were members of the genus Nitrincola (family Oceanospirillaceae, class Gammaproteobacteria). Strain ZV-19Tshowed 96.6 and 95.5{\%} sequence similarities and 19±3 and 18±3{\%}DNA-DNA relatedness to Nitrincola lacisaponensis DSM 16316Tand Nitrincola alkalisediminis JCM 19317T, respectively. Strains R4-8Tand S4-12 exhibited 97.9 and 98.6{\%}sequence matches and 34±4 and 13±8{\%}DNA-DNA hybridization values with N. lacisaponensis DSM 16316Tand N. alkalisediminis JCM 19317T, respectively. According to the phenotypic, chemotaxonomic and phylogenetic data, the strains studied represent two novel species, Nitrincola alkalilacustris sp. nov. with the type strain ZV-19T(=DSM 29817T=NCAIM B 02612T) and Nitrincola schmidtii sp. nov. with the type strain R4-8T (=DSM 100788T=NCAIM B.02626T). An emended description of the genus Nitrincola is also presented.",
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AU - Borsodi, A.

AU - Korponai, Kristóf

AU - Schumann, Peter

AU - Spröer, Cathrin

AU - Felföldi, Tamás

AU - Márialigeti, K.

AU - Szili-Kovács, Tibor

AU - Tóth, E.

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AB - Three alkaliphilic and halotolerant bacterial strains, designated ZV-19T, R4-8Tand S4-12, were isolated from the water of soda pans located in the Kiskunság National Park, Hungary. Cells of all three strains were Gram-staining-negative, rodshaped, motile and non-endospore-forming. They were facultatively anaerobic, and oxidase-and catalase-positive. Their major isoprenoid quinone was Q-8, and their predominant fatty acids were C18: 1ω7c, C16: 1ω7c and C16: 0. The DNA G+C content was 54.5 mol% in strain ZV-19 T and 45.8 mol% in strain R4-8T. The 16S rRNA gene based phylogenetic analysis showed that all three strains were members of the genus Nitrincola (family Oceanospirillaceae, class Gammaproteobacteria). Strain ZV-19Tshowed 96.6 and 95.5% sequence similarities and 19±3 and 18±3%DNA-DNA relatedness to Nitrincola lacisaponensis DSM 16316Tand Nitrincola alkalisediminis JCM 19317T, respectively. Strains R4-8Tand S4-12 exhibited 97.9 and 98.6%sequence matches and 34±4 and 13±8%DNA-DNA hybridization values with N. lacisaponensis DSM 16316Tand N. alkalisediminis JCM 19317T, respectively. According to the phenotypic, chemotaxonomic and phylogenetic data, the strains studied represent two novel species, Nitrincola alkalilacustris sp. nov. with the type strain ZV-19T(=DSM 29817T=NCAIM B 02612T) and Nitrincola schmidtii sp. nov. with the type strain R4-8T (=DSM 100788T=NCAIM B.02626T). An emended description of the genus Nitrincola is also presented.

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