Nitric oxide synthesis is blocked in the enternal nervous system during dormant periods of the snail Helix lucorum

Tamás Röszer, Zsolt Czimmerer, A. József Szentmiklósi, G. Bánfalvi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

During dormancy of terrestrial snails, the whole neuromodulation of the nervous system is deeply modified. In this work we studied the adaptation of a previously described, putatively nitric oxide (NO) forming enteral network to the long-term resting periods of the snail Helix lucorum. The standard NADPH diaphorase (NADPHd) technique, which is an accepted method for histochemical NO synthase (NOS) detection, labeled the same enteric neurons of the midintestine in active or hibernated snails. Quantification of the NO-derived nitrite by the Griess reaction established that the nitrite formation is confined to the NADPHd-reactive network containing the midintestinal segment. In active snails, the nitrite formation could be enhanced by the NOS substrate L-arginine (10 μM-1 mM), but decreased by the known NOS inhibitors 1 mM Nω-nitro-L- arginine (NOARG) and 10 mM aminoguanidine (AG). Application of 1 mM L-arginine and 1 mM NOARG decreased the amplitude of the midintestinal muscle contractile activity, but did not affect the rectal motility. In dormancy, the nitrite formation was reduced in the NADPHd-reactive midintestinal network. Application of L-arginine could not provoke nitrite production and did not influence the midintestinal motility. Our findings indicate that NO is involved in the neural transmission to intestinal muscles of gastropods, but enteric release of NO is blocked during dormancy. The decreased NO synthesis is possibly due to an as yet undefined mechanism, by which the L-arginine/NO conversion ability of NOS could temporarily be inhibited in the long-term resting period of H. lucorum.

Original languageEnglish
Pages (from-to)255-262
Number of pages8
JournalCell and Tissue Research
Volume316
Issue number2
DOIs
Publication statusPublished - May 2004

Fingerprint

Helix (Snails)
Neurology
Nervous System
Arginine
Nitrites
Nitric Oxide
NADPH Dehydrogenase
Nitric Oxide Synthase
Snails
Muscle
Muscles
Gastropoda
Synaptic Transmission
Neurons
Small Intestine
Substrates

Keywords

  • Enteric nervous system
  • Estivation
  • Helix lucorum L. (Mollusca)
  • Hibernation
  • NADPH diaphorase
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Nitric oxide synthesis is blocked in the enternal nervous system during dormant periods of the snail Helix lucorum. / Röszer, Tamás; Czimmerer, Zsolt; József Szentmiklósi, A.; Bánfalvi, G.

In: Cell and Tissue Research, Vol. 316, No. 2, 05.2004, p. 255-262.

Research output: Contribution to journalArticle

Röszer, Tamás ; Czimmerer, Zsolt ; József Szentmiklósi, A. ; Bánfalvi, G. / Nitric oxide synthesis is blocked in the enternal nervous system during dormant periods of the snail Helix lucorum. In: Cell and Tissue Research. 2004 ; Vol. 316, No. 2. pp. 255-262.
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