Nitric oxide synthase in healthy and inflamed human dental pulp

F. Di Nardo Di Maio, Zs. Lohinai, C. D'Arcangelo, P. Esposito De Fazio, L. Speranza, M. A. De Lutiis, A. Patruno, A. Grilli, M. Felaco

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Nitric oxide synthase (NOS) plays a significant role in the pathogenesis of pulpitis. In this study, we hypothesized the existence of endothelial (eNOS) and inducible (iNOS) enzyme isoforms in human dental pulp. Extracted third molar pulps were divided into groups based on clinical diagnosis: healthy, hyperemic, and irreversible pulpitis. We have localized the eNOS and iNOS by immunohistochemistry and have tested their mRNA expression by RT-PCR and protein levels by Western blots. eNOS is present in the endothelial cells and odontoblasts of the healthy pulp, but an elevation of eNOS mRNA and protein levels with a concomitant dilation of vessels was characteristic under pathological conditions. Healthy pulp tissue failed to exhibit any iNOS; however, acute inflammation enhanced the mRNA and protein levels of iNOS, mainly in the leukocytes. There are differences in localization and expression between eNOS and iNOS in healthy and inflamed dental pulp.

Original languageEnglish
Pages (from-to)312-316
Number of pages5
JournalJournal of Dental Research
Volume83
Issue number4
Publication statusPublished - Apr 2004

Fingerprint

Dental Pulp
Pulpitis
Nitric Oxide Synthase
Messenger RNA
Odontoblasts
Proteins
Third Molar
Dilatation
Protein Isoforms
Leukocytes
Endothelial Cells
Western Blotting
Immunohistochemistry
Inflammation
Polymerase Chain Reaction
Enzymes

Keywords

  • Endothelial cells
  • Human dental pulp
  • Inflammation
  • Nitric oxide synthase
  • Odontoblast

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Di Nardo Di Maio, F., Lohinai, Z., D'Arcangelo, C., Esposito De Fazio, P., Speranza, L., De Lutiis, M. A., ... Felaco, M. (2004). Nitric oxide synthase in healthy and inflamed human dental pulp. Journal of Dental Research, 83(4), 312-316.

Nitric oxide synthase in healthy and inflamed human dental pulp. / Di Nardo Di Maio, F.; Lohinai, Zs.; D'Arcangelo, C.; Esposito De Fazio, P.; Speranza, L.; De Lutiis, M. A.; Patruno, A.; Grilli, A.; Felaco, M.

In: Journal of Dental Research, Vol. 83, No. 4, 04.2004, p. 312-316.

Research output: Contribution to journalArticle

Di Nardo Di Maio, F, Lohinai, Z, D'Arcangelo, C, Esposito De Fazio, P, Speranza, L, De Lutiis, MA, Patruno, A, Grilli, A & Felaco, M 2004, 'Nitric oxide synthase in healthy and inflamed human dental pulp', Journal of Dental Research, vol. 83, no. 4, pp. 312-316.
Di Nardo Di Maio F, Lohinai Z, D'Arcangelo C, Esposito De Fazio P, Speranza L, De Lutiis MA et al. Nitric oxide synthase in healthy and inflamed human dental pulp. Journal of Dental Research. 2004 Apr;83(4):312-316.
Di Nardo Di Maio, F. ; Lohinai, Zs. ; D'Arcangelo, C. ; Esposito De Fazio, P. ; Speranza, L. ; De Lutiis, M. A. ; Patruno, A. ; Grilli, A. ; Felaco, M. / Nitric oxide synthase in healthy and inflamed human dental pulp. In: Journal of Dental Research. 2004 ; Vol. 83, No. 4. pp. 312-316.
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