Nitric oxide (NO) generation during vegetative/generative transition of the apical meristem in wheat

Zsuzsanna Kolbert, Andrea Petô, Réka Szôllôsi, L. Erdei, I. Tari

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The phase transition from vegetative to reproductive development of the shoot apical meristem (SAM) is a critical event during the life cycle of seed-propagated plants. Nitric oxide (NO) as a general plant signal plays a role during growth and development and under biotic and abiotic stress conditions. In this study, apical meristems of spring wheat (Triticum aestivum L. cv. Thaifun) were isolated during their development and NO was detected by DAF-FM using a fluorescent microscope. To test the NO specificity of DAF-FM, in vivo and in vitro experiments were also carried out. In vegetative apices NO level was very low and it significantly enhanced during transition (in the "double ridge" phase). After transition, NO level decreased and proved to be low in the generative phase (11 th-13 th week). Our results show that DAF-FM is a suitable and specific indicator of NO. The significant increase in NO level during the vegetative/generative transition of wheat SAM suggests its regulatory function.

Original languageEnglish
Pages (from-to)95-97
Number of pages3
JournalActa Biologica Szegediensis
Volume55
Issue number1
Publication statusPublished - 2011

Fingerprint

Meristem
apical meristems
Triticum
nitric oxide
Nitric Oxide
wheat
shoot meristems
Phase Transition
phase transition
biotic stress
Spermatophytina
Life Cycle Stages
spring wheat
Growth and Development
microscopes
abiotic stress
Seed
Life cycle
life cycle (organisms)
Seeds

Keywords

  • Nitric oxide
  • Shoot apical meristem
  • Vegetative/generative transition
  • Wheat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Nitric oxide (NO) generation during vegetative/generative transition of the apical meristem in wheat. / Kolbert, Zsuzsanna; Petô, Andrea; Szôllôsi, Réka; Erdei, L.; Tari, I.

In: Acta Biologica Szegediensis, Vol. 55, No. 1, 2011, p. 95-97.

Research output: Contribution to journalArticle

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