Nitric oxide is involved in the relaxant effect of capsaicin in the human sigmoid colon circular muscle

L. Barthó, R. Benkó, Z. Lázár, L. Illényi, Ä Horváth

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The relaxant effect of capsaicin (300 nM) has been studied on mucosa-free circular strips of the human sigmoid colon in vitro. The response of precontracted preparations to capsaicin (sub-maximal relaxation) was reduced by over 50% by the nitric oxide synthase inhibitor NG-nitro-L-arginine (L-NOARG; 20 μM or 100 μM) or by the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 1 μM), but not by tetrodotoxin (1 μM) or the P2 purinoceptor antagonist pyridoxal phosphate 6-azophenyl-2′,4′-disulfonic acid (PPADS; 50 μM). L-NOARG or ODQ caused moderate contraction of the circular muscle, indicating a tonic "nitrergic" control. Anandamide (1-100 μM), an endogenous cannabinoid and capsaicin VR1 receptor stimulant, failed to either mimic or modify the response to capsaicin (300 nM). It is proposed that capsaicin causes the release of smooth muscle relaxant substance(s) from afferent nerve endings in the gut wall, in a tetrodotoxin-resistant manner. Nitric oxide (possibly released from capsaicin-sensitive afferents) plays an important role in the capsaicin-evoked response. No evidence has been found for an involvement of PPADS- sensitive P2 purinoceptors in the response to capsaicin or for a stimulation or inhibition of capsaicin-sensitive receptors by anandamide in the human sigmoid colon.

Original languageEnglish
Pages (from-to)496-500
Number of pages5
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume366
Issue number5
DOIs
Publication statusPublished - Nov 11 2002

Keywords

  • Anandamide
  • Capsaicin
  • Human sigmoid colon
  • Nitric oxide
  • P purinoceptors

ASJC Scopus subject areas

  • Pharmacology

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